Rapid detection of antibiotic-resistant bacteria at the single-cell level using two-photon excitation fluorescence and coherent anti-Stokes Raman scattering microscopy (Conference Presentation)

Chi Zhang; Jungeun Won; Stephen A. Boppart

doi:10.1117/12.2545438

10 March 2020 Rapid detection of antibiotic-resistant bacteria at the single-cell level using two-photon excitation fluorescence and coherent anti-Stokes Raman scattering microscopy (Conference Presentation)

Chi Zhang, Jungeun Won, Stephen A. Boppart

Author Affiliations +

Proceedings Volume 11223, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020; 112230B (2020) https://doi.org/10.1117/12.2545438
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

The current standard for antibiotic susceptibility testing (AST) is based on measuring bacterial growth after 10-24 hours of proliferation. Considering that many life-threatening conditions of infection exist, rapid AST techniques are urgently needed. We developed a rapid AST method based on two-photon fluorescence and coherent anti-Stokes Raman scattering microscopy which can detect antibiotic responses of bacteria within one hour. We used Pseudomonas aeruginosa as a representative pathogen model, and found that antibiotic treatment greatly reduces nicotinamide adenine dinucleotide (phosphate) levels in the bacteria. This enables rapid determination of bacterial susceptibility at the single cell level.

Conference Presentation

Citation Download Citation

Chi Zhang, Jungeun Won, and Stephen A. Boppart "Rapid detection of antibiotic-resistant bacteria at the single-cell level using two-photon excitation fluorescence and coherent anti-Stokes Raman scattering microscopy (Conference Presentation)", Proc. SPIE 11223, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020, 112230B (10 March 2020); https://doi.org/10.1117/12.2545438

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Bacteria

CARS tomography

Luminescence

Microscopy

Resistance

Mode conditioning cables

Diagnostics

Show All Keywords

Keywords/Phrases

Search In:

Publication Years