High-throughput three-dimensional imaging cytometer for subnuclear foci quantification (Conference Presentation)

Cheng Zheng; Dushan N. Wadduwage; Jong Park; Christy Chao; Jenny Kay; Norah Owiti; Zachary Nagel; Bevin P. Engelward; Peter T. C. So

doi:10.1117/12.2546302

9 March 2020 High-throughput three-dimensional imaging cytometer for subnuclear foci quantification (Conference Presentation)

Cheng Zheng, Dushan N. Wadduwage, Jong Park, Christy Chao, Jenny Kay, Norah Owiti, Zachary Nagel, Bevin P. Engelward, Peter T. C. So

Author Affiliations +

Proceedings Volume 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII; 112430X (2020) https://doi.org/10.1117/12.2546302
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

We propose a high-throughput 3D imaging cytometer for fast quantification of DNA double strand break (DSB) frequency in cells for DNA damage study. With structured illumination enabled depth contrast and a fast focus tunable lens enabled scanning, this system generates a three-dimensional stack of clustered nuclei γH2AX foci with submicron resolution at a speed of 800 cells/second. Moreover, we unify the stack construction with the deep neural network, which largely improve quantification accuracy as well as the processing speed. Compared to previous 2D imaging approach, the addition of z-resolution in our 3D method provides an extra dimension of contrast and thus allows for more accurate DNA DSB quantification.

Conference Presentation

Citation Download Citation

Cheng Zheng, Dushan N. Wadduwage, Jong Park, Christy Chao, Jenny Kay, Norah Owiti, Zachary Nagel, Bevin P. Engelward, and Peter T. C. So "High-throughput three-dimensional imaging cytometer for subnuclear foci quantification (Conference Presentation)", Proc. SPIE 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII, 112430X (9 March 2020); https://doi.org/10.1117/12.2546302

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