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We present a Computational Miniature Mesoscope that enables 3D fluorescence imaging across an 8-mm field-of-view and 2.5-mm depth-of-field in a single shot, achieving 7-micrometer lateral resolution and better than 200-micrometer axial resolution. The mesoscope has a compact design that integrates a microlens array for imaging and an LED array for excitation on a single platform. Its expanded imaging capability is enabled by computational imaging. We experimentally validate the mesoscopic 3D imaging capability on volumetrically distributed fluorescent beads and fibers. We further quantify the effects of bulk scattering and background fluorescence on phantom experiments.
Yujia Xue,Ian G. Davison,David A. Boas, andLei Tian
"Large-scale high-resolution 3D fluorescence imaging with a computational miniature mesoscope", Proc. SPIE 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII, 1164903 (5 March 2021); https://doi.org/10.1117/12.2577667
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Yujia Xue, Ian G. Davison, David A. Boas, Lei Tian, "Large-scale high-resolution 3D fluorescence imaging with a computational miniature mesoscope," Proc. SPIE 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII, 1164903 (5 March 2021); https://doi.org/10.1117/12.2577667