Super-resolution acoustic-resolution photoacoustic microscopy by a novel algorithm

Fei Feng; Siqi Liang; Sung-Liang Chen

doi:10.1117/12.2574164

10 October 2020 Super-resolution acoustic-resolution photoacoustic microscopy by a novel algorithm

Fei Feng, Siqi Liang, Sung-Liang Chen

Proceedings Volume 11553, Optics in Health Care and Biomedical Optics X; 1155306 (2020) https://doi.org/10.1117/12.2574164
Event: SPIE/COS Photonics Asia, 2020, Online Only

Abstract

Acoustic-resolution photoacoustic microscopy (AR-PAM) is a promising tool for microvascular imaging. In AR-PAM, a focused transducer is typically used. Limited by acoustic diffraction, in-focus lateral resolution is dependent on the center frequency and numerical aperture of the transducer. On the other hand, out-of-focus lateral resolution will deteriorate, which can be restored to in-focus lateral resolution by synthetic aperture focusing technique (SAFT). Previously, we demonstrated that with prior knowledge of the point-spread function of the AR-PAM imaging system, combined SAFT and Richard-Lucy deconvolution can be applied to achieve super resolution (SR) beyond acoustic diffraction limit and to enhance signal-to-noise ratio (SNR) in both focal and out-of-focus regions. However, SNR of the original AR-PAM image highly affects the performance. Moreover, discontinuities arise in the line pattern that is originally continuous. In this study, we propose a novel algorithm, which combines a novel SAFT method and a directional model-based (D-M) deconvolution method, to break the acoustic diffraction limit. By using our algorithm, FWHM of 20 ~µm for AR-PAM system over DOF of ~1.8 mm is experimentally achieved. Compared with our previous work using Richard-Lucy deconvolution, the D-M deconvolution demonstrates the advantages in high SNR, and good line continuity. Compared with the directional SAFT method, our algorithm achieves SR and higher SNR.

Conference Presentation

Citation Download Citation

Fei Feng, Siqi Liang, and Sung-Liang Chen "Super-resolution acoustic-resolution photoacoustic microscopy by a novel algorithm", Proc. SPIE 11553, Optics in Health Care and Biomedical Optics X, 1155306 (10 October 2020); https://doi.org/10.1117/12.2574164

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