Interlaced two-photon volumetric scanning for measurement of capillary network dynamics

John Giblin; Anderson Chen; Seong-Wook Park; Kivilcim Kilic; John Jiang; David A. Boas

doi:10.1117/12.2578829

5 March 2021 Interlaced two-photon volumetric scanning for measurement of capillary network dynamics

John Giblin, Anderson Chen, Seong-Wook Park, Kivilcim Kilic, John Jiang, David A. Boas

Proceedings Volume 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics; 116290I (2021) https://doi.org/10.1117/12.2578829
Event: SPIE BiOS, 2021, Online Only

Abstract

We combined a home-built two photon microscope with a Bessel focus capable of volumetric imaging with a custom scan pattern that allowed us to generate image pairs with a time delay of ~12 ms. This approach allows us to track red blood cell (RBC) flow along individual capillaries and therefore calculate RBC speed. We demonstrate the ability to measure flow speeds in capillaries across a 475x475x150 µm volume which will enable us to monitor network wide changes in capillary flow.

Conference Presentation

Citation Download Citation

John Giblin, Anderson Chen, Seong-Wook Park, Kivilcim Kilic, John Jiang, and David A. Boas "Interlaced two-photon volumetric scanning for measurement of capillary network dynamics", Proc. SPIE 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 116290I (5 March 2021); https://doi.org/10.1117/12.2578829

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