Rapid Stokes imaging applied to monitor oscillations during optical tissue clearing

Jiawei Song; Nan Zeng; Hui Ma

doi:10.1117/12.2575044

5 March 2021 Rapid Stokes imaging applied to monitor oscillations during optical tissue clearing

Jiawei Song, Nan Zeng, Hui Ma

Proceedings Volume 11646, Polarized Light and Optical Angular Momentum for Biomedical Diagnostics; 1164615 (2021) https://doi.org/10.1117/12.2575044
Event: SPIE BiOS, 2021, Online Only

Conference Poster

Abstract

Tissue optical clearing techniques have become more and more prominent in biomedical applications, since they can reduce scattering and improve the imaging depth, resolution and contrast. Polarization is sensitive to the microstructure of tissues, when apply the rapid Stokes imaging method to monitor the process of optical tissue clearing, we observe that the polarization parameters oscillate periodically during clearing. A series of experiments have been designed to verify the truth of oscillations, we first eliminate the influence of system noise by measuring air and other polarization optics, the system error is less than 1% and is stable, then we analyze the impacts from optical clearing agents, we take quartz plate, the quartz plate with saturated sucrose solution, the fact is that there are slight fluctuations in polarization parameters but no oscillations. However, when we test the porcine skin which immersed in optical clearing agents, there are significant oscillations which show periodicity in polarization parameter, the oscillation cycle is about 40s and the oscillation amplitudes become smaller with the increase of immersion time. Finally, we imaging the samples which have completed optical clearing and no oscillation is found. By these series experiments, we confirm the truth of oscillations of the polarization parameters during clearing, and we believe these are connected with the mechanisms of the clearing which need further study.

Conference Presentation

Citation Download Citation

Jiawei Song, Nan Zeng, and Hui Ma "Rapid Stokes imaging applied to monitor oscillations during optical tissue clearing", Proc. SPIE 11646, Polarized Light and Optical Angular Momentum for Biomedical Diagnostics, 1164615 (5 March 2021); https://doi.org/10.1117/12.2575044

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