Rapid determination of antimicrobial susceptibility by stimulated Raman scattering imaging of D2O metabolic incorporation in a single bacterium

Meng Zhang; Weili Hong; Nader S. Abutaleb; Junjie Li; Pu-Ting Dong; Cheng Zong; Pu Wang; Mohamed Seleem; Ji-Xin Cheng

doi:10.1117/12.2575955

5 March 2021 Rapid determination of antimicrobial susceptibility by stimulated Raman scattering imaging of D₂O metabolic incorporation in a single bacterium

Meng Zhang, Weili Hong, Nader S. Abutaleb, Junjie Li, Pu-Ting Dong, Cheng Zong, Pu Wang, Mohamed Seleem, Ji-Xin Cheng

Author Affiliations +

Proceedings Volume 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021; 116560B (2021) https://doi.org/10.1117/12.2575955
Event: SPIE BiOS, 2021, Online Only

Abstract

This work demonstrates a rapid platform that can determine the antimicrobial susceptibility testing (AST) in cation-adjusted Mueller-Hinton Broth medium, urine and blood by stimulated Raman scattering (SRS) imaging of deuterium oxide (D2O) incorporation at a single bacterium level. The total AST assay time with the value of the single-cell metabolism inactivation concentration (SC-MIC) obtained is less than 2.5 h from colony to results. The SC-MIC results of 37 sets of bacterial isolate samples were systematically validated by MIC determined by the Clinical and Laboratory Standards Institute criteria, with a category agreement of 94.6% and 5.4% minor error. Furthermore, SRS imaging of D2O metabolic incorporation can rapidly determine SC-MIC directly in clinical samples for urinary tract infection or septicemia blood infection.

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Meng Zhang, Weili Hong, Nader S. Abutaleb, Junjie Li, Pu-Ting Dong, Cheng Zong, Pu Wang, Mohamed Seleem, and Ji-Xin Cheng "Rapid determination of antimicrobial susceptibility by stimulated Raman scattering imaging of D₂O metabolic incorporation in a single bacterium", Proc. SPIE 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021, 116560B (5 March 2021); https://doi.org/10.1117/12.2575955

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