PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
We present a straightforward approach to making sensitive fluorescence measurements using a chopped laser and phase-lock detection scheme. By using high-quality optical filters in the excitation and detection paths, the trade-off between spectral information and ultimate sensitivity is controlled by the choice of filter edge locations and bandwidths. Using this approach, we demonstrate a sensitivity of ~1 pW optical power of detected fluorescence over a bandwidth of ~125 nm centered at 505 nm using a 405 nm diode laser as an excitation source. We present the details of the measurement technique and discuss its value in selection of optical materials for fluorescence-based analytical systems.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
The alert did not successfully save. Please try again later.
Jeremy Grace, Samad Edlou, Joseph Foss, Craig Hodgson, Jean-Philippe Rheault, Jake Rosvold, Kurt Sieber, Sarah Walters, "High-sensitivity measurements of autofluorescence using optical interference filters," Proc. SPIE 11889, Optifab 2021, 118890C (28 October 2021); https://doi.org/10.1117/12.2602380