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24 March 2022 Brillouin spectroscopy imaging of cell phototoxic damage
Vsevolod Cheburkanov, Purboja Purkayastha, Kavya Pendyala, Tanmay Lele, Vladislav Yakovlev
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Proceedings Volume 11940, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic and Photobiomodulation Therapy XXX; 119400K (2022) https://doi.org/10.1117/12.2610498
Event: SPIE BiOS, 2022, San Francisco, California, United States
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Abstract
Recent advances in photodynamic inactivation and photobiomodulation require extensive research of application safety in living tissues in vitro and in vivo. Superficial phototoxicity induced cellular morphological changes have been observed and recorded with using confocal Brillouin microspectrometer. We are reporting evidence of biomechanical processes occurring in cells subjected to high-power laser radiation. 4T1 murine fibroblast cells were used in the study, making results easily after exposure to high power laser radiation. Spatial distribution of subcellular structures’ stiffness was recorded with high precision and analyzed, drawing correlation between existing morphological model and novel stiffness data within the cell.
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Vsevolod Cheburkanov, Purboja Purkayastha, Kavya Pendyala, Tanmay Lele, and Vladislav Yakovlev "Brillouin spectroscopy imaging of cell phototoxic damage", Proc. SPIE 11940, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic and Photobiomodulation Therapy XXX, 119400K (24 March 2022); https://doi.org/10.1117/12.2610498
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KEYWORDS
Spectroscopy
Imaging spectroscopy
Cancer
Confocal microscopy
High power lasers
Medical research
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