Efficient and versatile aberration correction through sensorless adaptive optics

Qi Hu; Martin Hailstone; Martin J. Booth

doi:10.1117/12.2610971

2 March 2022 Efficient and versatile aberration correction through sensorless adaptive optics

Qi Hu, Martin Hailstone, Martin J. Booth

Proceedings Volume 11966, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX; 1196605 (2022) https://doi.org/10.1117/12.2610971
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Wavefront-sensorless adaptive optics methods are often used to correct phase aberrations in optical systems and thus to improve imaging quality. However, sensorless methods have an intrinsic disadvantage of requiring multiple images that can lead to non-desirable photo-bleaching. We have proposed a machine learning assisted aberration correction method which could correct aberrations consisting of not fewer than five Zernike modes with as few as two images. We showed that our method could be used in microscopes to provide instant aberration predictions when imaging biological samples of non-specific structures. We showed that compared to conventional function fitting sensorless adaptive optics methods, the new method corrected much faster with observable advantages. This novel method has a great potential to be used in any adaptive optics equipped microscopy for efficient sensorless aberration correction for biomedical microscopy.

Conference Presentation

Citation Download Citation

Qi Hu, Martin Hailstone, and Martin J. Booth "Efficient and versatile aberration correction through sensorless adaptive optics", Proc. SPIE 11966, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX, 1196605 (2 March 2022); https://doi.org/10.1117/12.2610971

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available