Validation of Monro-Kellie doctrine during blood pressure lowering in mice using fNIRS

Hany Ferdinando; Jari Jukkola; Sadegh Moradi; Mika Kaakinen; Vesa Kiviniemi; Lauri Eklund; Teemu Myllylä

doi:10.1117/12.3005682

12 March 2024 Validation of Monro-Kellie doctrine during blood pressure lowering in mice using fNIRS

Hany Ferdinando, Jari Jukkola, Sadegh Moradi, Mika Kaakinen, Vesa Kiviniemi, Lauri Eklund, Teemu Myllylä

Proceedings Volume 12841, Dynamics and Fluctuations in Biomedical Photonics XXI; 1284106 (2024) https://doi.org/10.1117/12.3005682
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

The Monro-Kellie doctrine states that the sum of the contents of the intracranial cavity is constant, and consequently dynamics of blood and cerebrospinal fluid (CSF) volumes should be in an anti-correlation relationship. This phenomenon helped to explain many abnormalities in intracranial hypotension and CSF depletion. We aimed to validate the same phenomenon in mice during a blood pressure (BP) lowering test. Eight 2–3-month-old C57/Bl6N (Charles River) female mice were used in this study. We used both nicardipine hydrochloride and sodium nitroprusside (SNP) infusion into the femoral vein to lower the BP. A multi-wavelength NIRS (685, 830, and 980 nm) measuring hemoglobin and water concentrations, sampled at 800 Hz, was used. The fiber probes for the light source and detector were inserted into the ear canals and positioned towards the brain, giving a distance of approximately 1 cm. Following the Monro-Kellie doctrine, the blood volume, i.e., total hemoglobin (HbT), and CSF volume should be in an anti-correlation relationship. Our experiments showed that concentration changes of total hemoglobin (HbT) and water, are in anti-correlation with correlation coefficients of -0.991 ± 0.007.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Hany Ferdinando, Jari Jukkola, Sadegh Moradi, Mika Kaakinen, Vesa Kiviniemi, Lauri Eklund, and Teemu Myllylä "Validation of Monro-Kellie doctrine during blood pressure lowering in mice using fNIRS", Proc. SPIE 12841, Dynamics and Fluctuations in Biomedical Photonics XXI, 1284106 (12 March 2024); https://doi.org/10.1117/12.3005682

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