Optical Fourier analysis of biological cell patterns

Barry R. Masters

doi:10.1117/12.155714

3 September 1993 Optical Fourier analysis of biological cell patterns

Barry R. Masters

Proceedings Volume 1889, Holography, Interferometry, and Optical Pattern Recognition in Biomedicine III; (1993) https://doi.org/10.1117/12.155714
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

This paper presents the optical Fourier analysis of human corneal endothelial cell patterns. The quantitative analysis of human corneal endothelial cells patterns is analyzed with Fourier transform methods. The optical Fourier transform of the pattern of endothelial cell borders is analyzed to yield the average cell area and the coefficient of variation of the average cell area. The advantage of the Fourier transform analysis is the parallel processing of the optical Fourier transform and the potential development of a hybrid digital-optical device for the rapid analysis of large numbers of endothelial specular photomicrographs. The intensity distribution of the Fourier transform of binary images of endothelial cell patterns is detected with a wedge- ring detector. The radial and the angular distribution of intensity is analyzed and related to the cell size distribution in the input image.

Citation Download Citation

Barry R. Masters "Optical Fourier analysis of biological cell patterns", Proc. SPIE 1889, Holography, Interferometry, and Optical Pattern Recognition in Biomedicine III, (3 September 1993); https://doi.org/10.1117/12.155714

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