New reference compound with single, ultrashort lifetime for time-resolved tryptophan fluorescence experiments

Nina V. Visser; Antonie J. W. G. Visser; Tomas Konc; Pavel Kroh; Arie van Hoek

doi:10.1117/12.182707

17 August 1994 New reference compound with single, ultrashort lifetime for time-resolved tryptophan fluorescence experiments

Nina V. Visser, Antonie J. W. G. Visser, Tomas Konc, Pavel Kroh, Arie van Hoek

Author Affiliations +

Proceedings Volume 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV; (1994) https://doi.org/10.1117/12.182707
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

The time-correlated single photon counting technique in combination with ultrashort pulsed laser excitation is capable to determine picosecond fluorescence relaxation times provided that the data are accurately deconvoluted from the finite instrumental response which is typically tens of picoseconds. Knowledge of very rapid relaxation times in proteins is required to obtain insight into the role of structural fluctuations in protein function and to have an experimental tool to compare molecular dynamics simulations of proteins. We have found a reference compound having a short single fluorescence lifetime of 12 ps which is very useful for picosecond-resolved protein fluorescence spectroscopy. A description of some experimental pitfalls is given and the reference convolution method is illustrated with a few examples using N-acetyl-L-tryptophanamide.

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Nina V. Visser, Antonie J. W. G. Visser, Tomas Konc, Pavel Kroh, and Arie van Hoek "New reference compound with single, ultrashort lifetime for time-resolved tryptophan fluorescence experiments", Proc. SPIE 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV, (17 August 1994); https://doi.org/10.1117/12.182707

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