Incorporation-efficiency determination of the capsular plague antigen into liposomes based on elastic light scattering

V. V. Gusev; N. P. Guseva; S. N. Tatarintsev

doi:10.1117/12.200857

31 January 1995 Incorporation-efficiency determination of the capsular plague antigen into liposomes based on elastic light scattering

V. V. Gusev, N. P. Guseva, S. N. Tatarintsev

Author Affiliations +

Proceedings Volume 2326, Photon Transport in Highly Scattering Tissue; (1995) https://doi.org/10.1117/12.200857
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

In this paper the principle possibility for determining the incorporation efficiency of plague capsular antigen (PCA) into liposomes on the basis of elastic light scattering is shown. To find the most informative index, which characterizes the process of PCA sorption into liposomes, the angular light scattering dependencies for the liposome suspensions containing various PCA concentrations were measured by means of laser polarization nephelometer. Its construction and measurement technique are described in detail. For all preparations to be investigated, the incorporation efficiency was determined using traditional biochemical method and correlation between the scattering matrix element values and the incorporation efficiency was made. The most informative index, characterizing the process of PCA incorporation into the liposomes, appeared to be the light scattering integral intensity (LSII) at an angle of 90 degree(s). It was found that the PCA incorporation into liposomes results in the LSII increasing by a factor of 2 - 5 times in the incorporation efficiency range from 5% to 45%. The electron microscopy data and angular dependencies for the light scattering matrix elements have shown that LSII increase is due to the refraction index variation of the liposome membrane. The obtained LSII dependence on the incorporation efficiency enables us to determine the PCA incorporation efficiency into the liposomes by measuring the LSII.

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V. V. Gusev, N. P. Guseva, and S. N. Tatarintsev "Incorporation-efficiency determination of the capsular plague antigen into liposomes based on elastic light scattering", Proc. SPIE 2326, Photon Transport in Highly Scattering Tissue, (31 January 1995); https://doi.org/10.1117/12.200857

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