Whole bladder wall photodynamic therapy using 5-ALA: an experimental study in pigs

Hugo J. van Staveren; Johan F. Beek; Cess W.J. Verlaan; Annie Edixhoven; Anne E. Saarnak; Dick Sterenborg; Theo M. de Reijke; Guy Brutel de la Riviere; Sharon L. Thomsen M.D.; Martin J. C. van Gemert; Willem M. Star

doi:10.1117/12.230951

31 January 1996 Whole bladder wall photodynamic therapy using 5-ALA: an experimental study in pigs

Hugo J. van Staveren, Johan F. Beek, Cess W.J. Verlaan, Annie Edixhoven, Anne E. Saarnak, Dick Sterenborg, Theo M. de Reijke, Guy Brutel de la Riviere, Sharon L. Thomsen M.D., Martin J. C. van Gemert, Willem M. Star

Author Affiliations +

Proceedings Volume 2625, Photochemotherapy: Photodynamic Therapy and Other Modalities; (1996) https://doi.org/10.1117/12.230951
Event: BiOS Europe '95, 1995, Barcelona, Spain

Abstract

The agent 5-aminolevulinic acid (5-ALA) can be an alternative drug in whole bladder wall (WBW) photodynamic therapy (PDT), as its good tumor selectivity and the short time skin photosensitivity after systemic administration are advantageous for clinical use. To determine the maximum drug and light doses for reversible normal tissue damage, a pre-clinical study was performed using an in vivo normal piglet bladder model. First, the kinetics of PpIX production in 2 pigs was determined in vitro after oral administration of 75 and 150 mg/kg ALA respectively. The concentration of PpIX in plasma, and erythrocytes was determined by reversed phase high-performance liquid chromatography (HPLC) and the maximum was reached at approximately equals 5 hours after the administration of ALA. This provided a guideline for the optimum interval between ALA administration and light application. Next, various ALA doses were either administered orally or instilled in the bladder and different light doses were applied. Bladder biopsies were taken at regular intervals and normal tissue damage was investigated histologically. Reversible tissue damage was obtained using 60 mg/kg of 5-ALA in combination with a light dose of 100 J cm^-2 (non-scattered plus scattered 630 nm wavelength light) in the case of oral administration. In the case of intravesical instillation, a drug dose of 2.5 gram and a light dose of 100 J cm^-2 are still too high to obtain reversible tissue damage.

Citation Download Citation

Hugo J. van Staveren, Johan F. Beek, Cess W.J. Verlaan, Annie Edixhoven, Anne E. Saarnak, Dick Sterenborg, Theo M. de Reijke, Guy Brutel de la Riviere, Sharon L. Thomsen M.D., Martin J. C. van Gemert, and Willem M. Star "Whole bladder wall photodynamic therapy using 5-ALA: an experimental study in pigs", Proc. SPIE 2625, Photochemotherapy: Photodynamic Therapy and Other Modalities, (31 January 1996); https://doi.org/10.1117/12.230951

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available