Two-photon excitation laser scanning microscopy: applications in neuroscience

Winfried Denk

doi:10.1117/12.239549

10 May 1996 Two-photon excitation laser scanning microscopy: applications in neuroscience

Winfried Denk

Proceedings Volume 2678, Optical Diagnostics of Living Cells and Biofluids; (1996) https://doi.org/10.1117/12.239549
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

High resolution fluorescence imaging in intact tissues faces special challenges posed by scattering of excitation and fluorescence light and the need to avoid photodynamic damage. Significant improvements over conventional widefield and confocal imaging are provided when two-photon excitation is used. Applications to the functional imaging of the calcium dynamics in synaptic spines, small invertebrate neurites, and auditory hair cells are shown. Two-photon absorption induced photolysis can also be used for scanning photochemical microscopy and for high resolution measurements of diffusional coupling between cellular compartments.

Citation Download Citation

Winfried Denk "Two-photon excitation laser scanning microscopy: applications in neuroscience", Proc. SPIE 2678, Optical Diagnostics of Living Cells and Biofluids, (10 May 1996); https://doi.org/10.1117/12.239549

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