Indocyanine green in-situ administration and photothermal destruction of tumor cells using an 808-nm diode laser

Wei R. Chen; Claudia S. Phillips; Kenneth Eugene Bartels D.V.M.; Robert L. Adams; Robert E. Nordquist

doi:10.1117/12.239560

7 May 1996 Indocyanine green in-situ administration and photothermal destruction of tumor cells using an 808-nm diode laser

Wei R. Chen, Claudia S. Phillips, Kenneth Eugene Bartels D.V.M., Robert L. Adams, Robert E. Nordquist

Author Affiliations +

Proceedings Volume 2681, Laser-Tissue Interaction VII; (1996) https://doi.org/10.1117/12.239560
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

Laser tumor tissue photothermal interaction was studied using an 808 nm diode laser and indocyanine green as the photosensitizer. This in vitro study employed laser power in the range of 3 to 5 watts and an aqueous ICG solution that was administered to murine mammary tumor tissue by intralesional injection. Histology revealed a highly selective photothermal tumor tissue destruction at the center of the ICG injection, while the tumor cells not in the ICG area were spared. Also studied was the retention of photosensitizer in tissue following different methods of administration. The absorption spectra of tissue in the range of 400 to 900 nm were obtained at different time intervals after ICG injections for liver and kidney tissue, as well as tumor tissue. Our results showed, shortly after intracardiac injection, a rapid increase of ICG concentration in liver and virtually no accumulation of ICG in the subcutaneous tumor tissue. In contrast, the intratumoral-injected ICG remained in the tumor with sufficient concentration for a duration up to 48 hours, particularly in the case of well- circumscribed tumors. The combination of the in situ ICG administration and the 808-nm diode laser provided selective and controllable cancer tissue destruction when appropriate laser powers and dosage of ICG were employed.

Citation Download Citation

Wei R. Chen, Claudia S. Phillips, Kenneth Eugene Bartels D.V.M., Robert L. Adams, and Robert E. Nordquist "Indocyanine green in-situ administration and photothermal destruction of tumor cells using an 808-nm diode laser", Proc. SPIE 2681, Laser-Tissue Interaction VII, (7 May 1996); https://doi.org/10.1117/12.239560

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