ED50 determination and histological characterization of porcine dermal lesions produced by 1540-nm laser radiation pulses

Pedro J. Rico; Michael A. Mitchell; Thomas E. Johnson; William P. Roach

doi:10.1117/12.386278

17 May 2000 ED₅₀ determination and histological characterization of porcine dermal lesions produced by 1540-nm laser radiation pulses

Pedro J. Rico, Michael A. Mitchell, Thomas E. Johnson, William P. Roach

Author Affiliations +

Proceedings Volume 3907, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems X; (2000) https://doi.org/10.1117/12.386278
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

The military and industry are using 1540 nm laser systems for which current consensus safety standards are misleading. Threshold, ED₅₀, exposure data, along with mechanisms of laser-tissue interaction need to be more accurately determined. Recent studies within our group indicate the Yucatan mini-pig is a more applicable animal model for laser induced skin injury investigators. Laser delivery is accomplished using an Er:Glass system producing 1540 nm of light at millisecond exposure times and in the range of 17 to 77 J/cm². Dermal lesion development is evaluated for acute, 1 hour, and 24 hour post exposure presentation. Preliminary data obtained from dermal exposures indicate a difference in ED₅₀ for Yorkshire and Yucatan pigs. In the Yucatan mini-pig erythematous lesions are formed acutely while in the Yorkshire, lesions are seen at 24 hours. Preliminary data indicates that lesion development occurs at or near the basal layer of the epidermis causing nuclear pyknosis, cellular swelling and loss of cellular detail. Contrary to the theory that water absorption is the primary mechanism of dermal tissue damage observed from 1540 nm laser exposures, skin chromophores appears to play a role in lesion development.

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Pedro J. Rico, Michael A. Mitchell, Thomas E. Johnson, and William P. Roach "ED₅₀ determination and histological characterization of porcine dermal lesions produced by 1540-nm laser radiation pulses", Proc. SPIE 3907, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems X, (17 May 2000); https://doi.org/10.1117/12.386278

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