Fluorescence and reflectance spectra of freshly excised cervical tissue

Alex R. Zelenchuk; Esther Oliva; Howard Kaufman; Kevin T. Schomacker; Bizhan Bandarchi-Chamkhaleh; Jonathan D. Pitts

doi:10.1117/12.465229

1 May 2002 Fluorescence and reflectance spectra of freshly excised cervical tissue

Alex R. Zelenchuk, Esther Oliva, Howard Kaufman, Kevin T. Schomacker, Bizhan Bandarchi-Chamkhaleh, Jonathan D. Pitts

Proceedings Volume 4613, Optical Biopsy IV; (2002) https://doi.org/10.1117/12.465229
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Fluorescence emission and diffuse reflectance spectra of freshly excised cervical tissue were studied with two specially designed contact probes. The objective of the study was to reach a better understanding of the relationship between spectroscopic measurements and cervical tissue morphology. Tissue samples from loop electro-surgical excision and hysterectomy specimens were measured within 20 to 90 minutes of excision. Emission spectra with 337 nm excitation, and reflectance spectra were collected at wavelengths between 370 and 720 nm from different tissue sites. Hematoxylin-eosin stained slides of the measured zones were obtained and compared to the spectra. In one experiment, a contact probe with a central illumination fiber and two concentric rings of detection fibers (radii 0.1 and 1 mm), was placed in contact with the epithelium and used to measure spectra from ectocervix and endocervix. The influence of 5% acetic acid on fluorescence and reflectance spectra was also investigated. In another experiment, a single 100-micron fiber probe was placed perpendicular to a cut edge of tissue and scanned to measure spectra in depth. Depth scans were made over various areas of the cervix

Citation Download Citation

Alex R. Zelenchuk, Esther Oliva, Howard Kaufman, Kevin T. Schomacker, Bizhan Bandarchi-Chamkhaleh, and Jonathan D. Pitts "Fluorescence and reflectance spectra of freshly excised cervical tissue", Proc. SPIE 4613, Optical Biopsy IV, (1 May 2002); https://doi.org/10.1117/12.465229

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