Tissue clearing as a tool to enhance imaging capability for optical coherence tomography

Ruikang K. Wang

doi:10.1117/12.470494

14 June 2002 Tissue clearing as a tool to enhance imaging capability for optical coherence tomography

Ruikang K. Wang

Proceedings Volume 4619, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications VI; (2002) https://doi.org/10.1117/12.470494
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

The high scattering nature of non-transparent human tissue limits the imaging depth of optical coherence tomography (OCT) to 1-2 millimeters. By using the longer wavelength of the light source, the penetration depth is improved; the imaging contrast is however decreased largely due to the reduced backscattering in microscopic scale and the reduced refractive heterogeneity in macroscopic scale. For more effective diagnosis using OCT, a concurrent improvement of penetration depth and imaging contrast are often needed. We report in this paper that the OCT imaging depth and contrast can be enhanced concurrently by the use of osmotic agents. We demonstrate experimentally, by examples, that the topical applications of glycerol and propylene glycol, two common biocompatible and osmotically active solutions, onto the tissue surfaces could significantly improve the OCT imaging contrast and depth capability. The biotissues demonstrated include the rat skin, human oesophageal and gastric tissues.

Citation Download Citation

Ruikang K. Wang "Tissue clearing as a tool to enhance imaging capability for optical coherence tomography", Proc. SPIE 4619, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications VI, (14 June 2002); https://doi.org/10.1117/12.470494

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available