Effect of dehydration on optical clearing and OCT imaging contrast after impregnation of biological tissue with biochemical agents

Ruikang K. Wang; Yonghong He; Valery V. Tuchin

doi:10.1117/12.531389

1 July 2004 Effect of dehydration on optical clearing and OCT imaging contrast after impregnation of biological tissue with biochemical agents

Ruikang K. Wang, Yonghong He, Valery V. Tuchin

Author Affiliations +

Proceedings Volume 5316, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VIII; (2004) https://doi.org/10.1117/12.531389
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

There are two events happening simultaneously after the impregnation of tissue with the hyperosmotic agents. The diffusion of the agents into tissue reduces the refractive index mismatch between the scattering centres and background medium. And in the meantime, the hyperosmotic characteristics of agents cause tissue dehydration. Both processes are believed to have the effects on reducing light scattering of biological tissue. In order to investigate the role of dehydration in optical clearing effect on the tissue with the application of hyperosmotic agents, the skin tissues with the application of glycerol are dynamically imaged with optical coherence tomography (OCT). We demonstrate experimentally that both the depth and the contrast of OCT imaging of tissue can be enhanced by the application of agents. Higher concentration of agents causes more water loss of skin tissue and a stronger optical clearing effect. We suggest that the dehydration induced by the agents contributes to the enhancement of both OCT imaging depth and contrast. OCT imaging movies of optical clearing effect of hyperosmotic agents on the skin tissues in vitro are provided.

Citation Download Citation

Ruikang K. Wang, Yonghong He, and Valery V. Tuchin "Effect of dehydration on optical clearing and OCT imaging contrast after impregnation of biological tissue with biochemical agents", Proc. SPIE 5316, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VIII, (1 July 2004); https://doi.org/10.1117/12.531389

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