In vivo and intracellular sensing and imaging of free zinc ion

Rebecca A. Bozym; Hui-Hui Zeng; Michele Cramer; Andrea Stoddard; Carol A. Fierke; Richard B. Thompson

doi:10.1117/12.542329

1 July 2004 In vivo and intracellular sensing and imaging of free zinc ion

Rebecca A. Bozym, Hui-Hui Zeng, Michele Cramer, Andrea Stoddard, Carol A. Fierke, Richard B. Thompson

Author Affiliations +

Proceedings Volume 5318, Advanced Biomedical and Clinical Diagnostic Systems II; (2004) https://doi.org/10.1117/12.542329
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

We describe new methods for the study of zinc in biological specimens. Intracellular free zinc was determined at levels down to picomolar using an excitation ratiometric fluorescence-based biosensing approach using a carbonic anhydrase variant as transducer. A new fiber optic sensor suitable for in vivo use is also described using laser excitation and an emission ratiometric approach; the zinc concentration range of sensor response can be selected to fit the application.

Citation Download Citation

Rebecca A. Bozym, Hui-Hui Zeng, Michele Cramer, Andrea Stoddard, Carol A. Fierke, and Richard B. Thompson "In vivo and intracellular sensing and imaging of free zinc ion", Proc. SPIE 5318, Advanced Biomedical and Clinical Diagnostic Systems II, (1 July 2004); https://doi.org/10.1117/12.542329

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