In vivo tomographic imaging based on bioluminescence

Wenxiang Cong; Durairaj Kumar; Yubin Kang; Patrick Sinn; Earl Nixon; John Mienel Jr.; Melissa J. Suter; Lihong V. Wang; Geoffrey McLennan; Eric A. Hoffman; Ge Wang

doi:10.1117/12.560522

26 October 2004 In vivo tomographic imaging based on bioluminescence

Wenxiang Cong, Durairaj Kumar, Yubin Kang, Patrick Sinn, Earl Nixon, John Mienel Jr., Melissa J. Suter, Lihong V. Wang, Geoffrey McLennan, Eric A. Hoffman, Ge Wang

Author Affiliations +

Proceedings Volume 5535, Developments in X-Ray Tomography IV; (2004) https://doi.org/10.1117/12.560522
Event: Optical Science and Technology, the SPIE 49th Annual Meeting, 2004, Denver, Colorado, United States

Abstract

The most important task for bioluminescence imaging is to identify the emission source from the captured bioluminescent signal on the surface of a small tested animal. Quantitative information on the source location, geometry and intensity serves for in-vivo monitoring of infectious diseases, tumor growth, metastases in the small animal. In this paper, we present a point-spread function-based method for reconstructing the internal bioluminescent source from the surface light output flux signal. The method is evaluated for sensing the internal emission sources in nylon phantoms and within a live mouse. The surface bioluminescent signal is taken with a highly sensitive CCD camera. The results show the feasibility and efficiency of the proposed point-spread function-based method.

Citation Download Citation

Wenxiang Cong, Durairaj Kumar, Yubin Kang, Patrick Sinn, Earl Nixon, John Mienel Jr., Melissa J. Suter, Lihong V. Wang, Geoffrey McLennan, Eric A. Hoffman, and Ge Wang "In vivo tomographic imaging based on bioluminescence", Proc. SPIE 5535, Developments in X-Ray Tomography IV, (26 October 2004); https://doi.org/10.1117/12.560522

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