Multifocal multiphoton fluorescence lifetime microsocpy for biomedical applications

A. Deniset; S. Leveque-Fort; M. P. Fontaine-Aupart; G. Roger; P. Georges

doi:10.1117/12.632951

7 October 2005 Multifocal multiphoton fluorescence lifetime microscopy for biomedical applications

A. Deniset, S. Leveque-Fort, M. P. Fontaine-Aupart, G. Roger, P. Georges

Proceedings Volume 5860, Confocal, Multiphoton, and Nonlinear Microscopic Imaging II; 58600J (2005) https://doi.org/10.1117/12.632951
Event: European Conferences on Biomedical Optics 2005, 2005, Munich, Germany

Abstract

Two-photon microscopy is a key method for biological and medical research on cells and tissues mainly due to the submicronic spatial resolution. Unfortunately in its conventional form, this technique leads to long time recording for three-dimensional and fluorescence lifetime imaging because it requires a single point laser scanning. The most suitable way to improve acquisition time is to illuminate the biological sample with several excitation points simultaneously. We thus present a time-resolved multifocal multiphoton microscope. Besides the advantage of preserving biological samples by reducing by a factor 64 the exposition time, this method keeps also the possibility of measuring both intensity and lifetime images of the samples.

Citation Download Citation

A. Deniset, S. Leveque-Fort, M. P. Fontaine-Aupart, G. Roger, and P. Georges "Multifocal multiphoton fluorescence lifetime microscopy for biomedical applications", Proc. SPIE 5860, Confocal, Multiphoton, and Nonlinear Microscopic Imaging II, 58600J (7 October 2005); https://doi.org/10.1117/12.632951

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