Study on the autofluorescence profiles of iris pigment epithelium and retinal pigment epithetlium

Gaixia Xu; Junle Qu D.D.S.; Danni Chen; Yiwen Sun; Lingling Zhao; Ziyang Lin; Zhihua Ding; Hanben Niu

doi:10.1117/12.741571

1 May 2007 Study on the autofluorescence profiles of iris pigment epithelium and retinal pigment epithetlium

Gaixia Xu, Junle Qu D.D.S., Danni Chen, Yiwen Sun, Lingling Zhao, Ziyang Lin, Zhihua Ding, Hanben Niu

Proceedings Volume 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine; 65341N (2007) https://doi.org/10.1117/12.741571
Event: Fifth International Conference on Photonics and Imaging in Biology and Medicine, 2006, Wuhan, China

Abstract

Transplantation technique of retinal pigment epithelium has been noticeable in recent years and gradually put into clinical practice in treatment of retinal degenerative diseases. Generally, immunological, histochemical, and physical methods are used to study the iris pigment epithelium (IPE) and retinal pigment epithelium (RPE) cells, which need complex sample preparation. In this paper, we provided a simple autofluorescence microscopy to investigate the fresh porcine IPE and RPE cells without any pretreatment. The results showed that the morphology and size of both were similar, round and about 15 μm. The main flourophore in both cells was similar, i.e. lipofuscin. In additional, the autofluorescence spectrum of RPE shifted blue after light-induced damage by laser illuminating. Because it was easier for IPE to be damaged by laser than for RPE, and the power of one scanning operation to get a full image was strong enough to damage IPE sample, we hadn't get any satisfied autofluorescence spectrum of IPE.

Citation Download Citation

Gaixia Xu, Junle Qu D.D.S., Danni Chen, Yiwen Sun, Lingling Zhao, Ziyang Lin, Zhihua Ding, and Hanben Niu "Study on the autofluorescence profiles of iris pigment epithelium and retinal pigment epithetlium", Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 65341N (1 May 2007); https://doi.org/10.1117/12.741571

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