Effects of wavelength-dependent fluence attenuation on the noninvasive photoacoustic imaging of hemoglobin oxygen saturation in subcutaneous vasculature in vivo

Hao F. Zhang; Konstantin Maslov; Lihong V. Wang

doi:10.1117/12.761984

28 February 2008 Effects of wavelength-dependent fluence attenuation on the noninvasive photoacoustic imaging of hemoglobin oxygen saturation in subcutaneous vasculature in vivo

Hao F. Zhang, Konstantin Maslov, Lihong V. Wang

Author Affiliations +

Proceedings Volume 6856, Photons Plus Ultrasound: Imaging and Sensing 2008: The Ninth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics; 68561T (2008) https://doi.org/10.1117/12.761984
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Due to wavelength-dependent optical attenuation in the skin, the local fluence at a subcutaneous vessel varies with the optical wavelength in a spectral measurement. Hence compensation for such a spectral attenuation is necessary in quantitative measurements of the oxygen saturation of hemoglobin (sO₂) in blood vessels in vivo using photoacoustic (PA)imaging. Here, by employing a simplified double-layer skin model, we find that although the absolute value of sO₂ in a vessel is seriously affected by the volume fraction of blood and the spatially averaged sO2 in the dermis, the difference of sO₂ between neighboring vessels is minimally affected. Based on in vivo experiments, we demonstrate that the difference in sO₂ between a typical artery and a typical vein is conserved before and after an experimentally acquired spectral compensation. This conservation holds regardless of the animal's systemic physiological state.

Citation Download Citation

Hao F. Zhang, Konstantin Maslov, and Lihong V. Wang "Effects of wavelength-dependent fluence attenuation on the noninvasive photoacoustic imaging of hemoglobin oxygen saturation in subcutaneous vasculature in vivo", Proc. SPIE 6856, Photons Plus Ultrasound: Imaging and Sensing 2008: The Ninth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics, 68561T (28 February 2008); https://doi.org/10.1117/12.761984

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