A low conductivity culture medium suitable for the evaluation of sperm motility

Rui Ma; Chao Han; Zilong Sun; Guoliang Huang; Zhongyao Yu; Yuxiang Zhou; Jundong Wang; Jie Qiao; Jing Cheng

doi:10.1117/12.822761

6 March 2009 A low conductivity culture medium suitable for the evaluation of sperm motility

Rui Ma, Chao Han, Zilong Sun, Guoliang Huang, Zhongyao Yu, Yuxiang Zhou, Jundong Wang, Jie Qiao, Jing Cheng

Proceedings Volume 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine; 72800H (2009) https://doi.org/10.1117/12.822761
Event: Photonics and Optoelectronics Meetings, 2008, Wuhan, China

Abstract

A novel culture medium of low conductivity suitable for dielectrophoretic selection of sperm was developed. Conventional IVF methods lack the capability of selecting the expected sperms and the embryonic development may be adversely affected to certain extent. Dielectrophoresis (DEP), a technique commonly applied in cell manipulation [1], may provide an alternative. However, the conventional IVF medium has a high conductivity, which may result in the unexpected heating effect during DEP causing damage to the gametes. The newly developed medium consists of sucrose, HEPES, BSA and low concentrations of ions. The conductivity of this medium is significantly lower than the conventional IVF medium. Motility and membrane integrality of the mouse sperm were tested in the low-conductivity medium, demonstrating an acceptable percent rate of motile sperm compared to the control groups.

Citation Download Citation

Rui Ma, Chao Han, Zilong Sun, Guoliang Huang, Zhongyao Yu, Yuxiang Zhou, Jundong Wang, Jie Qiao, and Jing Cheng "A low conductivity culture medium suitable for the evaluation of sperm motility", Proc. SPIE 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine, 72800H (6 March 2009); https://doi.org/10.1117/12.822761

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