Gold nanorods for cell imaging with confocal reflectance microscopy and two-photon fluorescence microscopy

Ji-Yao Chen; Pei-Nan Wang

doi:10.1117/12.838703

24 February 2010 Gold nanorods for cell imaging with confocal reflectance microscopy and two-photon fluorescence microscopy

Ji-Yao Chen, Pei-Nan Wang

Author Affiliations +

Proceedings Volume 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII; 75680A (2010) https://doi.org/10.1117/12.838703
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Gold nanorods have unique optical properties as their two photon absorption cross sections are very high and their spectral positions of extinction bands can be controlled by their aspect ratio only, so that gold nanorods have been considered as agents for cell imaging. Two-photon photoluminescence imaging could be used to detect the cellular gold nanorods with the high power femto-second (fs) infrared laser, but may cause the photothermal effect melting the rods. The 3-D distribution of gold nanorods in living cells also can be measured by confocal reflectance microscopy with a very low laser power, and thus the cell damaging can be avoided. In this work, these two methods were comparatively studied in living rat basophilic leukemia (RBL-2H3) cells.

Citation Download Citation

Ji-Yao Chen and Pei-Nan Wang "Gold nanorods for cell imaging with confocal reflectance microscopy and two-photon fluorescence microscopy", Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 75680A (24 February 2010); https://doi.org/10.1117/12.838703

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