Photodynamic therapy on bacterial reduction in dental caries: in vivo study

Alessandra Baptista; Renato Araujo Prates; Ilka Tiemy Kato; Marcello Magri Amaral; Anderson Zanardi de Freitas; Martha Simões Ribeiro

doi:10.1117/12.854597

17 May 2010 Photodynamic therapy on bacterial reduction in dental caries: in vivo study

Alessandra Baptista, Renato Araujo Prates, Ilka Tiemy Kato, Marcello Magri Amaral, Anderson Zanardi de Freitas, Martha Simões Ribeiro

Author Affiliations +

Proceedings Volume 7715, Biophotonics: Photonic Solutions for Better Health Care II; 77151R (2010) https://doi.org/10.1117/12.854597
Event: SPIE Photonics Europe, 2010, Brussels, Belgium

Abstract

The reduction of pathogenic microorganisms in supragingival plaque is one of the principal factors in caries prevention and control. A large number of microorganisms have been reported to be inactivated in vitro by photodynamic therapy (PDT). The purpose of this study was to develop a rat model to investigate the effects of PDT on bacterial reduction in induced dental caries. Twenty four rats were orally inoculated with Streptococcus mutans cells (ATCC 25175) for three consecutive days. The animals were fed with a cariogenic diet and water with 10% of sucrose ad libitum, during all experimental period. Caries lesion formation was confirmed by Optical Coherence Tomography (OCT) 5 days after the beginning of the experiment. Then, the animals were randomly divided into two groups: Control Group: twelve animals were untreated by either light or photosensitizer; and PDT Group: twelve animals were treated with 100μM of methylene blue for 5min and irradiated by a Light Emitting Diode (LED) at λ = 640±30nm, fluence of 172J/cm², output power of 240mW, and exposure time of 3min. Microbiological samples were collected before, immediately after, 3, 7 and 10 days after treatment and the number of total microaerophiles was counted. OCT images showed areas of enamel demineralization on rat molars. Microbiological analysis showed a significant bacterial reduction after PDT. Furthermore, the number of total microaerophiles in PDT group remained lower than control group until 10 days posttreatment. These findings suggest that PDT could be an alternative approach to reduce bacteria in dental caries.

Citation Download Citation

Alessandra Baptista, Renato Araujo Prates, Ilka Tiemy Kato, Marcello Magri Amaral, Anderson Zanardi de Freitas, and Martha Simões Ribeiro "Photodynamic therapy on bacterial reduction in dental caries: in vivo study", Proc. SPIE 7715, Biophotonics: Photonic Solutions for Better Health Care II, 77151R (17 May 2010); https://doi.org/10.1117/12.854597

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available