Analysis of uncultured extremophilic snow algae by non-invasive single cell Raman spectroscopy

Thomas Beer; Zuki Tanaka; Nathan Netzter; Lynn J. Rothschild; Bin Chen

doi:10.1117/12.896481

23 September 2011 Analysis of uncultured extremophilic snow algae by non-invasive single cell Raman spectroscopy

Thomas Beer, Zuki Tanaka, Nathan Netzter, Lynn J. Rothschild, Bin Chen

Proceedings Volume 8152, Instruments, Methods, and Missions for Astrobiology XIV; 81520F (2011) https://doi.org/10.1117/12.896481
Event: SPIE Optical Engineering + Applications, 2011, San Diego, California, United States

Abstract

The study of life in extreme environments is a critical component of Astrobiology. But many of the so-called "extremophiles" are not readily cultivatable and therefore difficult to study under laboratory conditions. An example of such an extremophile is the snow alga Chlamydomonas cd. nivalis which expresses still unstudied secondary metabolites within its life cycle. In this paper, we present the first time the non-invasive single cell Raman spectroscopy of the life cycle dependent metabolite composition of C. nivalis. These secondary metabolites are likely related to the adaptation of C. nivalis to various stress factors. Normalized carotenoid Raman spectra intensities reveal characteristic ratio differences that allow identification of life cycle stages and putative secondary metabolites.

Citation Download Citation

Thomas Beer, Zuki Tanaka, Nathan Netzter, Lynn J. Rothschild, and Bin Chen "Analysis of uncultured extremophilic snow algae by non-invasive single cell Raman spectroscopy", Proc. SPIE 8152, Instruments, Methods, and Missions for Astrobiology XIV, 81520F (23 September 2011); https://doi.org/10.1117/12.896481

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