Mechanistic study of macrophage activation by LPS stimulation using fluorescence imaging techinques

Cuixia Lu; Feifan Zhou; Wei R. Chen; Da Xing

doi:10.1117/12.919009

13 March 2012 Mechanistic study of macrophage activation by LPS stimulation using fluorescence imaging techinques

Cuixia Lu, Feifan Zhou, Wei R. Chen, Da Xing

Proceedings Volume 8329, Tenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2011); 83290N (2012) https://doi.org/10.1117/12.919009
Event: Photonics and Optoelectronics Meetings 2011, 2011, Wuhan, China

Abstract

Lipopolysaccharide (LPS), a structural component of the outer membrane of gram negative bacteria, has been suggested that stimulates macrophages secrete a wide variety of inflammatory mediators, such as nitric oxide (NO). However, the cellular mechanisms of NO generation in macrophage by LPS stimulation are not well known. In this study, LPS stimulated NO generation in macrophage was determined by measuring fluorescence changes with a NO specific probe DAF-FM DA. Using the fluorescence resonance energy transfer (FRET) techniques, we found an increase of protein kinase C (PKC) activation was dynamically monitored in macrophages treated with LPS. Nuclear factor kappa B (NF-κB) translocated from the cytoplasm to the nucleus in macrophage was measured by confocal laser scanning microscopy. Moreover, the PKC inhibitor GÖ6983 inhibited LPS-stimulated NF-κB activation and NO production. These results indicated that LPS stimulated NF-κB mediated NO production by activating PKC.

Citation Download Citation

Cuixia Lu, Feifan Zhou, Wei R. Chen, and Da Xing "Mechanistic study of macrophage activation by LPS stimulation using fluorescence imaging techinques", Proc. SPIE 8329, Tenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2011), 83290N (13 March 2012); https://doi.org/10.1117/12.919009

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
10 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Luminescence

Fluorescence resonance energy transfer

NOx

Confocal microscopy

Confocal laser scanning microscopy

Bacteria

Microscopes

Show All Keywords

Keywords/Phrases

Search In:

Publication Years