ERK-dependent activation of Sp1 is required for low-power laser irradiation-induced vascular endothelial cell proliferation

Jie Feng; Da Xing

doi:10.1117/12.999836

11 December 2012 ERK-dependent activation of Sp1 is required for low-power laser irradiation-induced vascular endothelial cell proliferation

Jie Feng, Da Xing

Author Affiliations +

Proceedings Volume 8553, Optics in Health Care and Biomedical Optics V; 855332 (2012) https://doi.org/10.1117/12.999836
Event: Photonics Asia, 2012, Beijing, China

Abstract

Here, we report that low-power laser irradiation (LPLI) activates ERK/Sp1 pathway to upregulate VEGF expression and promote vascular endothelial cell proliferation. We demonstrate for the first time that LPLI enhances DNA-binding activity and transactivation activity of Sp1 on VEGF promoter. Additionally, ERK translocates from cytoplasm to nucleus following LPLI. Moreover, activated ERK phosphorylates Sp1 and results in increased EKR-Sp1 interaction. Selective inhibition of Sp1 or ERK suppresses the effect of LPLI on the promotion of cell cycle progression and proliferation. These findings provide a novel link between LPLI and angiogenesis, supplying potential therapy strategies for angiogenesis with LPLI.

Citation Download Citation

Jie Feng and Da Xing "ERK-dependent activation of Sp1 is required for low-power laser irradiation-induced vascular endothelial cell proliferation", Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 855332 (11 December 2012); https://doi.org/10.1117/12.999836

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