Diagnosis of basal cell carcinoma by two photon excited fluorescence combined with lifetime imaging

Shunping Fan; Xiao Peng; Lixin Liu; Shaoxiong Liu; Yuan Lu; Junle Qu

doi:10.1117/12.2038464

28 February 2014 Diagnosis of basal cell carcinoma by two photon excited fluorescence combined with lifetime imaging

Shunping Fan, Xiao Peng, Lixin Liu, Shaoxiong Liu, Yuan Lu, Junle Qu

Proceedings Volume 8948, Multiphoton Microscopy in the Biomedical Sciences XIV; 89482E (2014) https://doi.org/10.1117/12.2038464
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Basal cell carcinoma (BCC) is the most common type of human skin cancer. The traditional diagnostic procedure of BCC is histological examination with haematoxylin and eosin staining of the tissue biopsy. In order to reduce complexity of the diagnosis procedure, a number of noninvasive optical methods have been applied in skin examination, for example, multiphoton tomography (MPT) and fluorescence lifetime imaging microscopy (FLIM). In this study, we explored two-photon optical tomography of human skin specimens using two-photon excited autofluorescence imaging and FLIM. There are a number of naturally endogenous fluorophores in skin sample, such as keratin, melanin, collagen, elastin, flavin and porphyrin. Confocal microscopy was used to obtain structures of the sample. Properties of epidermic and cancer cells were characterized by fluorescence emission spectra, as well as fluorescence lifetime imaging. Our results show that two-photon autofluorescence lifetime imaging can provide accurate optical biopsies with subcellular resolution and is potentially a quantitative optical diagnostic method in skin cancer diagnosis.

Citation Download Citation

Shunping Fan, Xiao Peng, Lixin Liu, Shaoxiong Liu, Yuan Lu, and Junle Qu "Diagnosis of basal cell carcinoma by two photon excited fluorescence combined with lifetime imaging", Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 89482E (28 February 2014); https://doi.org/10.1117/12.2038464

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