Sub-diffraction-limited imaging by photobleaching imprinting microscopy

Liang Gao; Alejandro Garcia-Uribe; Yan Liu; Chiye Li; Lihong Wang

doi:10.1117/12.2038573

4 March 2014 Sub-diffraction-limited imaging by photobleaching imprinting microscopy

Liang Gao, Alejandro Garcia-Uribe, Yan Liu, Chiye Li, Lihong Wang

Proceedings Volume 8950, Single Molecule Spectroscopy and Superresolution Imaging VII; 895015 (2014) https://doi.org/10.1117/12.2038573
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

We present a generic sub-diffraction-limited imaging method – photobleaching imprinting microscopy (PIM) – for biological fluorescence imaging. A lateral resolution of 110 nm was measured, more than a two-fold improvement over the optical diffraction limit. Unlike other super-resolution imaging techniques, PIM does not require complicated illumination modules or specific fluorescent dyes. PIM is expected to facilitate the conversion of super-resolution imaging into a routine lab tool, making it accessible to a much broader biological research community.

Citation Download Citation

Liang Gao, Alejandro Garcia-Uribe, Yan Liu, Chiye Li, and Lihong Wang "Sub-diffraction-limited imaging by photobleaching imprinting microscopy", Proc. SPIE 8950, Single Molecule Spectroscopy and Superresolution Imaging VII, 895015 (4 March 2014); https://doi.org/10.1117/12.2038573

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