Visual detection of Akt mRNA in living cell using gold nanoparticle beacon

Yi Ma; Caiping Tian; Siwen Li; Zhaohui Wang; Yueqing Gu

doi:10.1117/12.2068691

17 September 2014 Visual detection of Akt mRNA in living cell using gold nanoparticle beacon

Yi Ma, Caiping Tian, Siwen Li, Zhaohui Wang, Yueqing Gu

Proceedings Volume 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014); 923009 (2014) https://doi.org/10.1117/12.2068691
Event: Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 2014, Wuhan, China

Abstract

PI3K-Akt signaling pathway plays the key role in cell apoptosis and survival, and the components of PI3K /Akt signaling pathway are often abnormally expressed in human tumors. Therefore, determination of the Akt (protein kinase B, PKB) messenger ribonucleic acid (mRNA) expression is significantly important in understanding the mechanism of tumor progression. In this study, we designed a special hairpin deoxyribonucleic acid (DNA) functionalized with gold nanoparticles and fluorescein isothiocyanate(FITC) as a beacon for detecting human Akt mRNA. Spectrofluorometer was used to detect the fluorescence quenching and recovery of the beacons, and laser confocal scanning microscopy was adopted to image Akt mRNA in cells. The results showed that this beacon could sensitively and quantitatively measure the Akt mRNA in living cells . This strategy is potentially useful for the cellular imaging of RNA or protein expression in living cells.

Citation Download Citation

Yi Ma, Caiping Tian, Siwen Li, Zhaohui Wang, and Yueqing Gu "Visual detection of Akt mRNA in living cell using gold nanoparticle beacon", Proc. SPIE 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 923009 (17 September 2014); https://doi.org/10.1117/12.2068691

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