Photoacoustic imaging of voltage signals
(Conference Presentation)

Bin Rao; Ruiying Zhang; Lihong V. Wang

doi:10.1117/12.2211747

27 April 2016 Photoacoustic imaging of voltage signals (Conference Presentation)

Bin Rao, Ruiying Zhang, Lihong V. Wang

Proceedings Volume 9708, Photons Plus Ultrasound: Imaging and Sensing 2016; 97081U (2016) https://doi.org/10.1117/12.2211747
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Optical imaging of brain voltage signals is significantly limited in depth due to optical scattering and the absorptive property of brain tissue. Photoacoustic (PA) imaging promises to break this hard limit by utilizing both ballistic and diffused photons. To demonstrate the feasibility of PA, we used an in vivo mouse model. The brain cortex tissue was stained with dipicrylamine dye, electrically stimulated, and imaged with a customized dual-isosbestic-wavelength PA microscope (DIW-PAM). DIW-PAM separates voltage-induced PA signals from blood-induced PA signals and thereby allows recording the voltage response of mouse cortex tissue without interference from hemoglobin responses. The resting state PA voltage response signal exhibited a noise-like signal in the frequency domain. Upon 3 Hz electrical stimulation, the PA voltage response signal showed frequency peaks of 3.2 Hz and 6.3 Hz (Fig. 1). Although dipicrylamine dye is not fast enough for recording neuron action potentials, it served well for the purpose of this feasibility study. In conclusion, we successfully demonstrated in vivo photoacoustic imaging of mouse brain voltage signals for the first time. If a fast voltage-sensitive dye is available, using photoacoustic computed tomography (PACT) instead of PA microscopy could allow acquiring full-field PA action potential images at a speed limited only by the laser pulse repetition rate.

Conference Presentation

Citation Download Citation

Bin Rao, Ruiying Zhang, and Lihong V. Wang "Photoacoustic imaging of voltage signals (Conference Presentation)", Proc. SPIE 9708, Photons Plus Ultrasound: Imaging and Sensing 2016, 97081U (27 April 2016); https://doi.org/10.1117/12.2211747

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Brain

Neuroimaging

Photoacoustic imaging

Action potentials

In vivo imaging

Photoacoustic tomography

Tissue optics

Show All Keywords

Keywords/Phrases

Search In:

Publication Years