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7 March 2022 Ultra-parallel label-free optophysiology of dynamic neural activity and circuit connectivity
Stephen A. Boppart
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Proceedings Volume PC11944, Multiscale Imaging and Spectroscopy III; PC119440E (2022) https://doi.org/10.1117/12.2617168
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
Neural activity patterns span over three orders-of-magnitude in both space and time. This presentation introduces a new versatile all-optical technique for neurophysiology that utilizes a novel label-free optical microscopy system called Superfast Polarization-sensitive Off-axis Full-field Optical Coherence Microscopy (SPoOF OCM). SPoOF OCM captures the changes to both the optical path length and birefringence induced by the millisecond-scale electrical activity of neurons. This multimodal response is imaged in a widefield configuration at 4000 frames per second, with a field-of-view of 200×200 µm^2, 1 µm transverse resolution, and 4.5 µm axial resolution. With an ability to capture responses spanning three orders-of-magnitude in both space and time, SPoOF OCM meets the exacting needs of a comprehensive neurophysiology tool, and overcomes existing limitations of traditional electrophysiology and fluorescence microscopy.
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Stephen A. Boppart "Ultra-parallel label-free optophysiology of dynamic neural activity and circuit connectivity", Proc. SPIE PC11944, Multiscale Imaging and Spectroscopy III, PC119440E (7 March 2022); https://doi.org/10.1117/12.2617168
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KEYWORDS
Electrophysiology
Birefringence
Ions
Luminescence
Microscopy
Neurons
Neurophysiology
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