Simultaneous functional and thermal imaging of mouse cortex

Hunter Banks; Shengxuan Chen; Jonathan Bumstead; Lindsey Brier; Annie Bice; Seana Gaines; Joseph Culver

doi:10.1117/12.2608942

28 April 2022 Simultaneous functional and thermal imaging of mouse cortex

Hunter Banks, Shengxuan Chen, Jonathan Bumstead, Lindsey Brier, Annie Bice, Seana Gaines, Joseph Culver

Proceedings Volume PC11946, Neural Imaging and Sensing 2022; PC119460W (2022) https://doi.org/10.1117/12.2608942
Event: SPIE BiOS, 2022, San Francisco, California, United States

Conference Poster

Abstract

Obtaining high signal to noise ratio is challenging in wide-field two photon microscopy and one must ensure the mouse brain can be imaged safely under high laser power. Here, we demonstrated a simultaneous thermal imaging and two photon imaging technique. The maximum temperature of the cortex was below 39°C using 400mW laser power with a 5 x 5mm field of view. Together with the brain activities under hind paw stimulation and at rest, we argued that high laser power for wide-field two-photon imaging can potentially be used while keeping the temperature under safety limit.

Conference Presentation

Citation Download Citation

Hunter Banks, Shengxuan Chen, Jonathan Bumstead, Lindsey Brier, Annie Bice, Seana Gaines, and Joseph Culver "Simultaneous functional and thermal imaging of mouse cortex", Proc. SPIE PC11946, Neural Imaging and Sensing 2022, PC119460W (28 April 2022); https://doi.org/10.1117/12.2608942

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