Raman micro-spectroscopy reveals the spatial distribution of fumarate in living cells (Conference Presentation)

Marlous Kamp; Jakub Surmacki; Vincent Zecchini; Karolina Chrabaszcz; Marc S. Mondejar; Tim Young; Christian Frezza; Sarah E. Bohndiek

doi:10.1117/12.2648523

15 March 2023 Raman micro-spectroscopy reveals the spatial distribution of fumarate in living cells (Conference Presentation)

Marlous Kamp, Jakub Surmacki, Vincent Zecchini, Karolina Chrabaszcz, Marc S. Mondejar, Tim Young, Christian Frezza, Sarah E. Bohndiek

Author Affiliations +

Proceedings Volume PC12383, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI; PC1238308 (2023) https://doi.org/10.1117/12.2648523
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Mutations in fumarate hydratase (FH) lead to the accumulation of fumarate, considered to be an ‘oncometabolite’ due to the wide-ranging consequences of its accumulation in the cell. Here, we demonstrate that Raman spectroscopy (RS) can detect and map fumarate in living cells. We highlight the presence of three main peaks at 1281±2cm-1, 1403±2cm-1 and 1657±2cm-1. In live cells, we measure an average fumarate concentration of 10 mM in FH-proficient cells compared to 23 mM for FH-deficient cells, in agreement with prior mass spectrometry measurements. Future studies will focus on enhancing sensitivity using coherent or surface enhanced Raman methods.

Conference Presentation

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Marlous Kamp, Jakub Surmacki, Vincent Zecchini, Karolina Chrabaszcz, Marc S. Mondejar, Tim Young, Christian Frezza, and Sarah E. Bohndiek "Raman micro-spectroscopy reveals the spatial distribution of fumarate in living cells (Conference Presentation)", Proc. SPIE PC12383, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI, PC1238308 (15 March 2023); https://doi.org/10.1117/12.2648523

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Raman spectroscopy

Mass spectrometry

Mode conditioning cables

Remote sensing

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