Quantitative analysis of dehydration in porcine skin for assessing mechanism of optical clearing

Tingting Yu; Xiang Wen; Qingming Luo; Dan Zhu; Valery V. Tuchin

doi:10.1117/1.3621515

1 September 2011 Quantitative analysis of dehydration in porcine skin for assessing mechanism of optical clearing

Tingting Yu, Xiang Wen, Qingming Luo, Dan Zhu, Valery V. Tuchin

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Journal of Biomedical Optics, Vol. 16, Issue 9, 095002 (September 2011). https://doi.org/10.1117/1.3621515

Abstract

Dehydration induced by optical clearing agents (OCAs) can improve tissue optical transmittance; however, current studies merely gave some qualitative descriptions. We develop a model to quantitatively evaluate water content with partial least-squares method based on the measurements of near-infrared reflectance spectroscopy and weight of porcine skin. Furthermore, a commercial spectrometer with an integrating sphere is used to measure the transmittance and reflectance of skin after treatment with different OCAs, and then the water content and optical properties of sample are calculated, respectively. The results show that both the reduced scattering coefficient and dehydration of skin decrease with prolongation of action of OCAs, but the relative change in former is larger than that in latter after a 60-min treatment. The absorption coefficient at 1450 nm decreases completely coincident with dehydration of skin. Further analysis illustrates that the correlation coefficient between the relative changes in the reduced scattering coefficient and dehydration is ∼1 during the 60-min treatment of agents, but there is an extremely significant difference between the two parameters for some OCAs with more hydroxyl groups, especially, glycerol or D-sorbitol, which means that the dehydration is a main mechanism of skin optical clearing, but not the only mechanism.

©(2011) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Tingting Yu, Xiang Wen, Qingming Luo, Dan Zhu, and Valery V. Tuchin "Quantitative analysis of dehydration in porcine skin for assessing mechanism of optical clearing," Journal of Biomedical Optics 16(9), 095002 (1 September 2011). https://doi.org/10.1117/1.3621515

Published: 1 September 2011

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KEYWORDS

Skin

Optical clearing

Scattering

Water

Reflectivity

Absorption

Refractive index

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