Label-free real-time imaging of myelination in the Xenopus laevis tadpole by in vivo stimulated Raman scattering microscopy

Chun-Rui Hu; Delong Zhang; Mikhail N. Slipchenko; Ji-Xin Cheng; Bing Hu

doi:10.1117/1.JBO.19.8.086005

7 August 2014 Label-free real-time imaging of myelination in the Xenopus laevis tadpole by in vivo stimulated Raman scattering microscopy

Chun-Rui Hu, Delong Zhang, Mikhail N. Slipchenko, Ji-Xin Cheng, Bing Hu

Author Affiliations +

Journal of Biomedical Optics, Vol. 19, Issue 8, 086005 (August 2014). https://doi.org/10.1117/1.JBO.19.8.086005

Abstract

The myelin sheath plays an important role as the axon in the functioning of the neural system, and myelin degradation is a hallmark pathology of multiple sclerosis and spinal cord injury. Electron microscopy, fluorescent microscopy, and magnetic resonance imaging are three major techniques used for myelin visualization. However, microscopic observation of myelin in living organisms remains a challenge. Using a newly developed stimulated Raman scattering microscopy approach, we report noninvasive, label-free, real-time in vivo imaging of myelination by a single-Schwann cell, maturation of a single node of Ranvier, and myelin degradation in the transparent body of the Xenopus laevis tadpole.

Citation Download Citation

Chun-Rui Hu, Delong Zhang, Mikhail N. Slipchenko, Ji-Xin Cheng, and Bing Hu "Label-free real-time imaging of myelination in the Xenopus laevis tadpole by in vivo stimulated Raman scattering microscopy," Journal of Biomedical Optics 19(8), 086005 (7 August 2014). https://doi.org/10.1117/1.JBO.19.8.086005

Published: 7 August 2014

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

CITATIONS

Cited by 24 scholarly publications.

Explore citations on Lens.org

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Axons

In vivo imaging

Microscopy

Nerve

Signal processing

Imaging systems

Image segmentation

Show All Keywords

RELATED CONTENT

Assessment of neuroglial relationships under photodynamic treatment using fluorescent visualization...
Proceedings of SPIE (January 30 2014)

Automated peripheral nerve tissue classification based on PS OCT derived...
Proceedings of SPIE (January 01 1900)

Label-free cancer cell death monitoring by stimulated Raman microscopy
Proceedings of SPIE (January 01 1900)

Convolutional neural network denoising in fluorescence lifetime imaging microscopy (FLIM)
Proceedings of SPIE (March 05 2021)

An improved U Net for nerve fibre segmentation in confocal...
Proceedings of SPIE (March 10 2020)

Optical coherence tomography of the rat cavernous nerves
Proceedings of SPIE (March 23 2007)

Subscribe to Digital Library

Receive Erratum Email Alert

Show All Keywords

Keywords/Phrases

Search In:

Publication Years