High-throughput ultraviolet photoacoustic microscopy with multifocal excitation

Toru Imai; Junhui Shi; Terence T. W. Wong; Lei Li; Liren Zhu; Lihong V. Wang

doi:10.1117/1.JBO.23.3.036007

15 March 2018 High-throughput ultraviolet photoacoustic microscopy with multifocal excitation

Toru Imai, Junhui Shi, Terence T. W. Wong, Lei Li, Liren Zhu, Lihong V. Wang

Author Affiliations +

Journal of Biomedical Optics, Vol. 23, Issue 3, 036007 (March 2018). https://doi.org/10.1117/1.JBO.23.3.036007

Abstract

Ultraviolet photoacoustic microscopy (UV-PAM) is a promising intraoperative tool for surgical margin assessment (SMA), one that can provide label-free histology-like images with high resolution. In this study, using a microlens array and a one-dimensional (1-D) array ultrasonic transducer, we developed a high-throughput multifocal UV-PAM (MF-UV-PAM). Our new system achieved a 1.6 ± 0.2 μm lateral resolution and produced images 40 times faster than the previously developed point-by-point scanning UV-PAM. MF-UV-PAM provided a readily comprehensible photoacoustic image of a mouse brain slice with specific absorption contrast in ∼16 min, highlighting cell nuclei. Individual cell nuclei could be clearly resolved, showing its practical potential for intraoperative SMA.

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Toru Imai, Junhui Shi, Terence T. W. Wong, Lei Li, Liren Zhu, and Lihong V. Wang "High-throughput ultraviolet photoacoustic microscopy with multifocal excitation," Journal of Biomedical Optics 23(3), 036007 (15 March 2018). https://doi.org/10.1117/1.JBO.23.3.036007

Received: 8 December 2017; Accepted: 22 February 2018; Published: 15 March 2018

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