Prof. Alessandra N. de Souza Rastelli Profile

Prof. Alessandra N. de Souza Rastelli

Professor at Univ. Estadual Paulista "Júlio de Mesquita Filho"

SPIE Involvement:

Conference Program Committee | Author

Proceedings Article | 10 March 2020 Presentation

Photodynamic inactivation using curcumin-loaded Pluronic® F-127 over Streptococcus mutans biofilm (Conference Presentation)

Alessandra Nara de Souza Rastelli, Diego Dantas Lopes dos Santos, João Felipe Bessegato, Joao Augusto Oshiro, Marlus Chorilli, Vanderlei Bagnato

Proceedings Volume 11223, 112230J (2020) https://doi.org/10.1117/12.2545296

KEYWORDS: Light emitting diodes, Photodynamic therapy, Microorganisms, Polymers, Dynamic light scattering, Transmission electron microscopy, Confocal microscopy

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Proceedings Article | 14 August 2019 Presentation

Antimicrobial photodynamic therapy mediated by chlorin e-6 photosensitizer against Streptococcus mutans biofilm (Conference Presentation)

Alessandra Nara de Souza Rastelli, Adilson César Abreu Bernardi, Camila Rodero, Marlus Chorilli, Kléber Thiago de Oliveira, Dongmei Deng, Vanderlei Bagnato

Proceedings Volume 11070, 110700N (2019) https://doi.org/10.1117/12.2528235

KEYWORDS: Photodynamic therapy, Dental caries, Mode conditioning cables, Statistical analysis, Picosecond phenomena, Brain, Heart, Confocal laser scanning microscopy, Confocal microscopy, Biological research

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Proceedings Article | 4 March 2019 Presentation

Effect of photodynamic inactivation associated with ultrasound on Streptococcus mutans biofilm (Conference Presentation)

Alessandra Nara de Souza Rastelli, Priscila Borges Gobbo de Melo, Jessica Katarine de Abreu Silva, João Felipe Besegato, Vanderlei Salvador Bagnato

Proceedings Volume 10863, 108630X (2019) https://doi.org/10.1117/12.2509695

KEYWORDS: Ultrasonography, Bacteria, Dental caries, Teeth, Natural surfaces, Organisms, Photodynamic therapy, Photosensitizer targeting, Tissues, Light emitting diodes

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Dental biofilms are produced by bacterial communities. During the first 24 h of colonization, oral streptococci compose 60% to 90% of the supragingival plaque biomass. Mutans streptococci are biofilm-forming bacteria and are considered to be the primary etiologic agents of human dental caries. They possess a variety of abilities to colonize tooth surfaces and under certain conditions are present in large quantities in cariogenic biofilms and also form biofilms with other organisms, including other streptococci and bacteria. To reduce microbial, like biofilm, can be performed by using photodynamic therapy. Successful of this kind of therapy is induced by penetration of light and photosensitizer into target cells. The sonodynamic therapy offers greater penetrating capability into tissues. Then, the purpose of this study was to evaluate the antibacterial effect of photodynamic inactivation (PDI) associated with ultrasound (U) using curcumin solution irradiated by LED light source over Streptococcus mutans biofilm. Initially, minimum inhibitory (MIC) and minimum bactericidal (MBC) concentrations were performed, The concentrations of 40 and 80 μM were selected for the next experiments. Streptococcus mutans biofilm were induced using a 96-well plates for 7 days according to Groups G1 (negative control, L0D0), G2 (L + U +), G3 (L + D0), G4 (L0D40-80), G5 (chlorhexidine, positive control), G6 (L + D40-80 U +), G7 (L + D40-80) and G8 (D40-80 U +). For the dark cytotoxicity, curcumin was incubated for 5 minutes. For PDI, the groups were incubated in the dark for 5minutes (pre-irradiation time) and irradiated by blue LED at 15J/cm2 (36mW/cm2) for 7minutes and 55seconds. Ultrasound was used for 5min under 0.16KW of power output and 1.5A (pre-irradiation). After treatment, the strains were seeded on BHI agar and incubated at 37°C for 48 hours to determine the number of CFU/mL. The results were transformed into log10 and submitted to analysis of variance (ANOVA) and Tukey test at the 5% level. Significant reductions in the number of viable cells of S. mutans were observed in groups G6 (6log10) providing 4log10 of bacterial reduction when compared to group G1 (2log10) (p <0.05). The association of PDI and ultrasound can be an effective method to control microorganisms in the oral cavity, especially S. mutans, which causes dental caries.

Proceedings Article | 14 March 2018 Presentation

Photodynamic inactivation (PDI) over cariogenic biofilm using photosensitizers based on curcumin (Conference Presentation)

Alessandra Nara de Souza Rastelli, Adilson César de Abreu Bernardi, Sebastião Pratavieira, Kléber Thiago de Oliveira, Dongmei Deng, Vanderlei Salvador Bagnato, Marlus Chorilli

Proceedings Volume 10479, 104790W (2018) https://doi.org/10.1117/12.2289775

KEYWORDS: Dental caries, Light emitting diodes, Statistical analysis, Confocal microscopy, Bacteria, Confocal laser scanning microscopy, Absorption, Luminescence

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Proceedings Article | 1 August 2017 Paper

Effectiveness of partially soluble photosensitizer in photodynamic microbiological inactivation: a curcumin example

Sebastião Pratavieira, Fatima Matroodi, Fabio Francisco Pinto-Júnior, Alessandra Nara Souza Rastelli, Vanderlei Bagnato, Francisco E. Guimarães

Proceedings Volume 10417, 104170R (2017) https://doi.org/10.1117/12.2282147

KEYWORDS: Confocal microscopy, Microscopes, Luminescence

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Proceedings Article | 5 March 2013 Paper

Study on the Curcumin dynamics and distribution through living biofilms

Mariana Carvalho, Lívia Dovigo, Alessandra N. Rastelli, Vanderlei Bagnato

Proceedings Volume 8569, 85690A (2013) https://doi.org/10.1117/12.2005306

KEYWORDS: Picosecond phenomena, Microorganisms, Luminescence, Confocal microscopy, Fluorescence lifetime imaging, Microscopes, Pathogens, Dental caries, Resistance, Surface plasmons

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Proceedings Article | 1 February 2012 Paper

Diffusion analysis of one photosensitizer in bovine teeth using fluorescence optical imaging

S. Montanha, S. Pratavieira, D. Jacomassi, A. N. S. Rastelli, V. Bagnato

Proceedings Volume 8208, 82080Q (2012) https://doi.org/10.1117/12.909136

KEYWORDS: Picosecond phenomena, Diffusion, Luminescence, Photoacoustic tomography, Photodynamic therapy, Teeth, CCD cameras, Optical imaging, Diamond, Image processing

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Proceedings Article | 30 January 2012 Paper

Optical characterization of one dental composite resin using bovine enamel as reinforcing filler

J. T. Tribioli, D. Jacomassi, A. N. S. Rastelli, S. Pratavieira, V. S. Bagnato, C. Kurachi

Proceedings Volume 8208, 82080R (2012) https://doi.org/10.1117/12.909195

KEYWORDS: Composite resins, Teeth, Luminescence, Particles, Reflectivity, Dentistry, Composites, Optical properties, Surface plasmons, Charged particle optics

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Showing 5 of 8 publications

Conference Committee Involvement (6)

Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2025

25 January 2025 | San Francisco, California, United States

Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2024

29 January 2024 | San Francisco, California, United States

Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2023

28 January 2023 | San Francisco, California, United States

Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2022

21 February 2022 | San Francisco, California, United States

Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2021

6 March 2021 | Online Only, California, United States

Showing 5 of 6 Conference Committees

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