Total internal reflection fluorescence (TIRF) microscopy is a well-known technique allowing to confine the light close to the surface of a glass substrate. This axial confinement is based on the generation of evanescent waves. In TIRF microscopy however, there is no control of the light intensity in the transverse plane. Here, we propose a method to create evanescent patterns, which uses a fast-switching digital micro-mirror device to generate and scan an evanescent spot at multiple positions on the sample plane. In this way, patterns confined in the three dimensions of space can be produced in a fraction of second. This method would allow better spatial control in photo-activation or photo-conversion experiments in living cells, e.g. to target processes located at cell membranes.
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