Dr. Asaf Biber Profile

Dr. Asaf Biber

at Chaim Sheba Medical Ctr

SPIE Involvement:

Author

Publications (1)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 13 March 2024 Presentation

Saliva-based extraction-free molecular assay for rapid diagnostics of SARS-CoV-2

Michael Margulis, Hanan Rohana, Oran Erster, Michal Mandelboim, Asaf Biber, Eli Schwartz, Avi Peretz, Amos Danielli

Proceedings Volume PC12861, PC1286108 (2024) https://doi.org/10.1117/12.3008270

KEYWORDS: Diagnostics, Viruses, Gold, Polymerase chain reaction, Modulation, COVID 19, Biosensing

Read Abstract +

The COVID-19 pandemic has emphasized the inability of diagnostic laboratories' testing capacity to keep up with the surging demand. The primary reasons were the lack of reagents (e.g., viral transport media and nucleic acid extraction kits) and the low throughput of the gold-standard molecular detection method (RT-qPCR). While the reagent shortages were eventually resolved, the limited throughput of the RT-qPCR remains a bottleneck for high-throughput testing applications even today. Here, we introduce a rapid saliva-based extraction-free molecular assay, which utilizes a non-invasive saliva sampling and extraction-free sample preparation, a fast endpoint RT-PCR and a high-throughput optical modulation biosensing (ht-OMBi) detection platform. We blindly tested 364 paired nasopharyngeal swabs and saliva samples from suspected SARS-CoV-2 cases in Israel. Compared with the gold standard swab-based RT-qPCR, the presented assay's sensitivity and specificity are 90.7% and 95.3%, respectively, but is achieved with only 50 min. sample-to-result turnaround time (~60% faster than the regular RT-qPCR), allowing high throughput and considerable savings of the reagents and disposables.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years