Dr. Bertrand Simon Profile

Dr. Bertrand Simon

Assistant Professor at Institut d’Optique Graduate School

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Author

Publications (10)

Proceedings Article | 30 March 2020 Presentation + Paper

Mirror-effect based tomographic diffraction microscopy

Nicolas Verrier, Ludovic Foucault, Matthieu Debailleul, Jean-Baptiste Courbot, Bruno Colicchio, Bertrand Simon, Laurent Vonna, Olivier Haeberlé

Proceedings Volume 11351, 1135106 (2020) https://doi.org/10.1117/12.2559200

KEYWORDS: Microscopy, Tomography, Diffraction

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Proceedings Article | 30 March 2020 Presentation + Paper

Curved microdisplay, from optical design to mechanical study: impact on form-factor and light efficiency in visual systems

S. Charrière, P. Joly, B. Simon, S. Nicolas, F. Zuber, D. Henry, O. Haeberle

Proceedings Volume 11350, 1135006 (2020) https://doi.org/10.1117/12.2552758

KEYWORDS: Optical design, Visual system, Organic light emitting diodes, Monochromatic aberrations, Gallium nitride, Visualization, Image quality, Sensors

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Proceedings Article | 16 July 2019 Paper

A simplified approach for tomographic diffractive microscopy

L. Foucault, N. Verrier, M. Debailleul, B. Simon, O. Haeberlé

Proceedings Volume 11172, 111720F (2019) https://doi.org/10.1117/12.2521798

KEYWORDS: Digital holography, Diffraction, Time division multiplexing, Mirrors, Optical transfer functions, Tomography, Demodulation, Microscopy, Reflectivity, Refractive index

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Proceedings Article | 24 May 2018 Presentation + Paper

Simplified approach for tomographic diffractive microscopy of axisymmetric samples

L. Foucault, N. Verrier, M. Debailleul, B. Simon, O. Haeberlé

Proceedings Volume 10677, 106771F (2018) https://doi.org/10.1117/12.2305730

KEYWORDS: Tomography, Microscopy, Diffraction, 3D acquisition, Fourier transforms, 3D image processing

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Proceedings Article | 29 April 2016 Paper

3D high- and isotropic resolution in tomographic diffractive microscopy by illumination angular scanning, specimen rotation and improved data recombination

Jonathan Bailleul, Bertrand Simon, Bruno Colicchio, Matthieu Debailleul, Olivier Haeberlé

Proceedings Volume 9896, 98960M (2016) https://doi.org/10.1117/12.2227058

KEYWORDS: Image resolution, Time division multiplexing, Spatial resolution, 3D image processing, Image resolution, 3D acquisition, Digital holography, Microscopes, 3D displays

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Proceedings Article | 26 April 2016 Paper

Overview of label-free far field optical nanoscopy techniques for nanometrology

Paul Montgomery, Audrey Leong-Hoï, Freddy Anstotz, Hui Liu, Bertrand Simon, Matthieu Debailleul, Olivier Haeberlé

Proceedings Volume 9890, 98900G (2016) https://doi.org/10.1117/12.2227582

KEYWORDS: Super resolution microscopy, Microscopy, Super resolution, Nanostructures, Optical imaging, Image resolution, Molecules, Digital holography, Nanoparticles, Confocal microscopy, Time division multiplexing, Tomography, Deconvolution, Optical microscopy

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Proceedings Article | 2 May 2012 Paper

GPU acceleration towards real-time image reconstruction in 3D tomographic diffractive microscopy

J. Bailleul, B. Simon, M. Debailleul, H. Liu, O. Haeberlé

Proceedings Volume 8437, 843707 (2012) https://doi.org/10.1117/12.922147

KEYWORDS: Visualization, Holograms, 3D image processing, 3D image reconstruction, Microscopy, 3D acquisition, Tomography, Time division multiplexing, Computer architecture, Image restoration

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Proceedings Article | 2 May 2008 Paper

Biological samples observed with diffractive tomographic microscopy

B. Simon, M. Debailleul, V. Georges, O. Haeberlé, V. Lauer

Proceedings Volume 6991, 699112 (2008) https://doi.org/10.1117/12.781154

KEYWORDS: Tomography, Microscopes, Microscopy, Refraction, Luminescence, Diffraction, Holography, 3D acquisition, Objectives, Confocal microscopy

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Proceedings Article | 22 April 2008 Paper

Recent advances in 3-D fluorescence microscopy: tomography as a source of information

A. Dieterlen, M. Debailleul, A. De Meyer, B. Simon, V. Georges, B. Colicchio, O. Haeberlé, V. Lauer

Proceedings Volume 7008, 70080S (2008) https://doi.org/10.1117/12.796995

KEYWORDS: Point spread functions, Luminescence, Microscopes, Microscopy, Deconvolution, Tomography, 3D image processing, Image processing, 3D acquisition, Optical microscopy

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3-D optical fluorescent microscopy becomes now an efficient tool for volume investigation of living biological samples. Developments in instrumentation have permit to beat off the conventional Abbe limit, in any case the recorded image can be described by the convolution equation between the original object and the Point Spread Function (PSF) of the acquisition system. If the goal is 3-D quantitative analysis, whether you improve the instrument capabilities, or (and) you restore the data. These last is until now the main task in our laboratory. Based on the knowledge of the optical Transfer Function of the microscope, deconvolution algorithms were adapted to automatic determine the regularisation threshold in order to give less subjective and more reproducible results. The PSF represents the properties of the image acquisition system; we have proposed the use of statistical tools and Zernike moments to describe a 3-D system PSF and to quantify the variation of the PSF. This first step toward standardization is helpful to define an acquisition protocol optimizing exploitation of the microscope depending on the studied biological sample. We have pointed out that automating the choice of the regularization level; if it facilitates the use, it also greatly improves the reliability of the measurements. Furthermore, to increase the quality and the repeatability of quantitative measurements a pre-filtering of images improves the stability of deconvolution process. In the same way, the PSF pre-filtering stabilizes the deconvolution process. We have shown that Zernike polynomials can be used to reconstruct experimental PSF, preserving system characteristics and removing the noise contained in the PSF. Fluorescent microscopes suffer from limitations; photobleaching and phototoxicity effects, or influence of the sample optical properties to 3-D observation. Amplitude and phase of the object can be reached with optical tomography based on a combination of microholography with a tomographic illumination. So indices cartography of the specimen can be obtained, and combined with fluorescence information it will open new possibilities in 3-D optical microscopy.

Proceedings Article | 10 September 2004 Paper

The point-spread function of fluorescence microscope imaging through an inhomogeneous medium

Olivier Haeberle, Bertrand Simon

Proceedings Volume 5462, (2004) https://doi.org/10.1117/12.545792

KEYWORDS: Point spread functions, Microscopes, Glasses, Refraction, Interfaces, Confocal microscopy, Luminescence, Microscopy, Diffraction, Water

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Showing 5 of 10 publications

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