Mr. Bo Gao Profile

Bo Gao

at South China Normal Univ

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Publications (2)

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Proceedings Article | 27 October 2006 Paper

Detection of irradiation induced reactive oxygen species production in live cells

Bo Gao, Debin Zhu

Proceedings Volume 6047, 604738 (2006) https://doi.org/10.1117/12.710703

KEYWORDS: Oxygen, Helium neon lasers, Luminescence, Cell death, Laser irradiation, Microscopes, Ultraviolet radiation, Laser scanners, Tumors, Hydrogen

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Reactive oxygen species (ROS) is thought to play an important role in cell signaling of apoptosis, necrosis, and proliferation. Light irradiation increases mitochondrial reactive oxygen species (ROS) production and mediates its intracellular signaling by adjusting the redox potential in tumor cells. Mitochondria are the main source of ROS in the living cell. Superoxide anions (0₂^- are likely the first ROS generated in the mitochondria following radiation damage, and then convert to hydrogen peroxide (H₂0₂), hydroxyl radical (•OH), and singlet oxygen (¹0₂), etc. Conventional methods for research ROS production in mitochondria mostly use isolated mitochondria rather than mitochondria in living cells. In this study, a highly selective probe to detect mitochondrial 0₂^- in live cells, MitoSOX^TM Red, was applied to quantify the mitochondrial ROS production in human lung adenocarcinoma cells (ASTC-a-1) with laser scanning microscope (LSM) after ultraviolet C (UVC) and He-Ne laser irradiation. Dichiorodihydrofluoresein diacetate (DCFHDA), a common used fluorescent probe for ROS detection without specificity, were used as a comparison to image the ROS production. The fluorescent image of MItoSOX^TM Red counterstained with MitoTracker Deep Red 633, a mitochondria selective probe, shows that the mitochondrial ROS production increases distinctly after UVC and He-Ne laser irradiation. DCFH-DA diffuses labeling throughout the cell though its fluorescence increases markedly too. In conclusion, the fluorescent method with MitoSOX^TM Red reagent is proved to be a promising technique to research the role of ROS in radiation induced apoptosis.

Proceedings Article | 14 February 2005 Paper

Evaluation of UVA-induced oxidative stress using a highly sensitive chemiluminescence method

Bo Gao, Da Xing, Debin Zhu

Proceedings Volume 5634, (2005) https://doi.org/10.1117/12.572940

KEYWORDS: Chemiluminescence, Oxygen, Ultraviolet radiation, Blood, Organisms, Radiotherapy, Signal detection, Signal processing, Proteins, Biological research

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Oxidative stress is mainly mediated by reactive oxygen species (ROS). Evaluation of oxidative stress is helpful for choosing an appropriate method to protect the organism from the oxidative damage. In this study, a highly sensitive and simple chemiluminescence method is presented for the evaluation of radiation-induced oxidative stress in human peripheral lymphocytes. The lymphocytes were irradiated by ultraviolet radiation (320-400nm, UVA) with different doses. The ROS generated by the lymphocytes was detected by chemiluminescence method, using a highly sensitive chemiluminescence probe 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-α] pyrazin-3-one (MCLA). The cell viability was detected with Cell Counting Kit-8 (CCK-8). The malondialdehyde (MDA), a marker of lipid peroxidation and oxidative stress, and the total antioxidant capacity (TAC), a parameter that is taken as evidence of oxidative stress, were measured too. The results show that both chemiluminescence intensity, cell mortality and MDA concentration of lymphocytes grow with the increase of UVA dose range from 0.5 to 8 J/cm², while the TAC decreases. There exists a positive relationship between cell oxidative damage degree and the chemiluminescence intensity of lymphocytes. This highly sensitive chemiluminescence method would potentially provide an easy way to evaluate the level of UVA-induced oxidative stress readily, sensitively and rapidly

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