Prof. Christopher W. Dunsby Profile

Prof. Christopher W. Dunsby

Professor in Biomedical Optics at Imperial College London

SPIE Involvement:

Author

Publications (36)

Proceedings Article | 15 March 2023 Presentation + Paper

Oblique plane microscopy for 3D, multi-color, eight-day time-lapse imaging of genetically heterogeneous breast cancer spheroids in 384-well plates with quantitative 3D analysis

Leo Rowe-Brown, Nathan Curry, Sandesh Meyler, Nina Moderau, Chris Dunsby

Proceedings Volume 12383, 1238302 (2023) https://doi.org/10.1117/12.2648952

KEYWORDS: Image segmentation, 3D image processing, Time lapse microscopy, Fluorescence, Breast cancer, 3D modeling, Fluorescent proteins, Cancer, Biological imaging, Machine learning, Live cell imaging

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Proceedings Article | 11 January 2023 Presentation

High-speed and high-content light-sheet fluorescence microscopy

Christopher Dunsby

Proceedings Volume 12333, 1233302 (2023) https://doi.org/10.1117/12.2657926

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Proceedings Article | 7 December 2021 Paper

Single-shot quantitative phase contrast using polarisation-resolved differential phase microscopy

R. Kalita, J. Lightley, S. Kumar, Y. Alexandrov, E. Garcia, W. Flanagan, M. A. Neil, C. Dunsby, P. M. French

Proceedings Volume 11922, 119220Y (2021) https://doi.org/10.1117/12.2615736

KEYWORDS: Microscopy, Phase contrast, LCDs, Cameras, Polarizers, Microscopes, Luminescence, Optical filters, Objectives, Image acquisition

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Proceedings Article | 7 December 2021 Paper

Robust optical autofocus system utilizing neural networks applied to automated multiwell plate STORM microscopy

J. Lightley, F. Görlitz, S. Kumar, R. Kalita, A. Kolbeinsson, E. Garcia, Y. Alexandrov, V. Bousgouni, R. Wysoczanski, P. Barnes, L.. Donelly, C. Bakal, C. Dunsby, M. A. Neil, S. Flaxman, P. M. French

Proceedings Volume 11922, 1192205 (2021) https://doi.org/10.1117/12.2615663

KEYWORDS: Microscopes, Data acquisition, Microscopy, Data modeling, Imaging systems, Objectives, Neural networks, Infrared radiation, Infrared imaging, Cameras

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Proceedings Article | 9 March 2020 Presentation

Oblique plane microscope plate reader for time lapse 3D imaging of live cells in collagen (Conference Presentation)

Nathan Curry, Hugh Sparks, Lucas Dent, Vicky Bousgouni, Vincent Maioli, Ranjeet Kumar, Sunil Kumar, Chris Bakal, Christopher Dunsby

Proceedings Volume 11243, 112430Z (2020) https://doi.org/10.1117/12.2542691

KEYWORDS: Microscopes, Stereoscopy, Collagen, Cancer, Microscopy, Imaging systems, Luminescence, Fluorescence spectroscopy, Spatial resolution, Data acquisition

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Proceedings Article | 3 March 2020 Paper

Towards easier, faster, super-resolved microscopy

Frederik Görlitz, Riccardo Wysoczanski, Sunil Kumar, Jonathan Lightley, Edwin Garcia, Yuriy Alexandrov, Ian Munro, Simon Johnson, Martin Kehoe, Callum Hollick, Jeremy Graham, Louise Donnelly, Peter Barnes, Christopher Dunsby, Mark Neil, Paul M. French

Proceedings Volume 11246, 112460R (2020) https://doi.org/10.1117/12.2550682

KEYWORDS: Microscopy, Microscopes, Stimulated emission depletion microscopy, Spatial light modulators, Objectives, Luminescence, Holograms, Data processing, Point spread functions, Data acquisition

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Proceedings Article | 2 March 2020 Paper

FLIM, FRET and high content analysis

Edwin Garcia, Wenjun Guo, Sunil Kumar, Frederik Görlitz, Hugh Sparks, Yuriy Alexandrov, Ian Munro, Douglas Kelly, Sean Warren, George Chennell, Alessandro Sardini, David Carling, Peter Thorpe, Christopher Dunsby, Paul M. French

Proceedings Volume 11244, 1124417 (2020) https://doi.org/10.1117/12.2547517

KEYWORDS: Fluorescence lifetime imaging, Fluorescence resonance energy transfer, Biosensors, Proteins, Luminescence, Data acquisition, Confocal microscopy, Fluorescent proteins, Microscopes, Biological research

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Proceedings Article | 22 July 2019 Paper

Automated multiwell plate STORM: towards open source super-resolved high content analysis

F. Görlitz, J. Lightley, S. Kumar, E. Garcia, M. Yan, R. Wysoczanski, Y. Alexandrov, J. Baker, P. Barnes, I. Munro, L. Donnelly, C. Dunsby, M. A. Neil, P. M. French

Proceedings Volume 11076, 1107605 (2019) https://doi.org/10.1117/12.2526940

KEYWORDS: Microscopy, Data processing, Data acquisition, Imaging systems, Microscopes

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Proceedings Article | 27 April 2016 Presentation

Three-dimensional fluorescence imaging by stage-scanning oblique plane microscopy (Conference Presentation)

Vincent Maioli, Frederik Görlitz, Sean Warren, Sunil Kumar, Paul M. French, George Chennell, Alessandro Sardini, David Carling, Frederike Alwes, Christopher Dunsby

Proceedings Volume 9713, 971318 (2016) https://doi.org/10.1117/12.2208808

KEYWORDS: 3D image processing, Microscopy, 3D acquisition, Luminescence, Microscopes, Cameras, Fluorescence resonance energy transfer, Stereoscopy, Objectives, Tumors

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Proceedings Article | 19 November 2014 Paper

Nanoparticle-assisted STED, theory, and experimental demonstration (presentation video)

Yannick Sonnefraud, Yonatan Sivan, Hugo Sinclair, Christopher Dunsby, Mark Neil, Paul French, Stefan Maier

Proceedings Volume 9169, 916903 (2014) https://doi.org/10.1117/12.2060993

KEYWORDS: Stimulated emission depletion microscopy, Video, Metals, Nanoparticles, Super resolution, Luminescence, Particles, Nanoimaging, Nanospectroscopy, Current controlled current source

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Proceedings Article | 17 March 2014 Paper

Autofluorescence lifetime metrology for label-free detection of cartilage matrix degradation

Mohammad Nickdel, João Lagarto, Douglas Kelly, Hugh Manning, Kazuhiro Yamamoto, Clifford Talbot, Christopher Dunsby, Paul French, Yoshifumi Itoh

Proceedings Volume 8940, 89400H (2014) https://doi.org/10.1117/12.2037285

KEYWORDS: Cartilage, Collagen, Tissues, Fluorescence lifetime imaging, Luminescence, Fluorometers, Scanning electron microscopy, Microscopy, Metrology, Microscopes

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Proceedings Article | 16 June 2011 Paper

Application of oblique plane microscopy to high speed live cell imaging

Sunil Kumar, Dean Wilding, Markus Sikkel, Alexander Lyon, Ken MacLeod, Chris Dunsby

Proceedings Volume 8086, 80860V (2011) https://doi.org/10.1117/12.889841

KEYWORDS: Microscopy, Tissue optics, Microscopes, Luminescence, Objectives, Live cell imaging, Oxygen, 3D image processing, Imaging systems, Cameras

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Proceedings Article | 14 June 2011 Paper

Non-invasive imaging of skin cancer with fluorescence lifetime imaging using two photon tomography

Rakesh Patalay, Clifford Talbot, Yuriy Alexandrov, Ian Munro, Hans Georg Breunig, Karsten König, Sean Warren, Mark Neil, Paul M. French, Anthony Chu, Gordon Stamp, Christopher Dunsby

Proceedings Volume 8087, 808718 (2011) https://doi.org/10.1117/12.889314

KEYWORDS: Luminescence, Fluorescence lifetime imaging, Skin, Tissues, Hyperspectral imaging, Diagnostics, In vivo imaging, Spectroscopy, Skin cancer, Image segmentation

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Proceedings Article | 1 March 2011 Paper

An automated wide-field time-gated optically sectioning fluorescence lifetime imaging multiwell plate reader for high-content analysis of protein-protein interactions

Dominic Alibhai, Sunil Kumar, Douglas Kelly, Sean Warren, Yuriy Alexandrov, Ian Munro, James McGinty, Clifford Talbot, Edward Murray, Frank Stuhmeier, Mark A. Neil, Chris Dunsby, Paul M. French

Proceedings Volume 7904, 79041Q (2011) https://doi.org/10.1117/12.875135

KEYWORDS: Fluorescence resonance energy transfer, Fluorescence lifetime imaging, Luminescence, Proteins, Data modeling, Picosecond phenomena, Image segmentation, Rhodamine B, Plasma, Biological research

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Proceedings Article | 1 March 2011 Paper

A multispectral FLIM tomograph for in-vivo imaging of skin cancer

C. Talbot, R. Patalay, I. Munro, H. Breunig, K. König, Y. Alexandrov, S. Warren, A. Chu, G. Stamp, M. A. Neil, Paul M. French, Christopher Dunsby

Proceedings Volume 7903, 79032B (2011) https://doi.org/10.1117/12.873567

KEYWORDS: Sensors, Fluorescence lifetime imaging, Skin, Luminescence, In vivo imaging, Image segmentation, Spectroscopy, Image sensors, Detection and tracking algorithms, Hyperspectral imaging

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Proceedings Article | 1 March 2011 Paper

A STED-FLIM microscope applied to imaging the natural killer cell immune synapse

M. Lenz, A. C. Brown, E. Auksorius, D. Davis, C. Dunsby, M. A. Neil, P. M. French

Proceedings Volume 7903, 79032D (2011) https://doi.org/10.1117/12.875018

KEYWORDS: Stimulated emission depletion microscopy, Fluorescence lifetime imaging, Microscopes, Confocal microscopy, Microscopy, Luminescence, Spatial resolution, Sapphire lasers, Structured optical fibers, Spatial light modulators

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Proceedings Article | 1 March 2011 Paper

Optically sectioned imaging by oblique plane microscopy

Sunil Kumar, Ziduo Lin, Alex Lyon, Ken MacLeod, Chris Dunsby

Proceedings Volume 7904, 79040X (2011) https://doi.org/10.1117/12.873471

KEYWORDS: Microscopy, Microscopes, Luminescence, Tissue optics, Objectives, Image processing, Imaging systems, Heart, 3D acquisition, Mirrors

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Proceedings Article | 22 February 2011 Paper

Fluorescence lifetime imaging endoscopy

G. Kennedy, S. Coda, A. Thompson, D. Elson, M. A. Neil, G. Stamp, A. Thillainayagam, B. Viellerobe, F. Lacombe, C. Dunsby, Paul M. French

Proceedings Volume 7893, 789308 (2011) https://doi.org/10.1117/12.874968

KEYWORDS: Fluorescence lifetime imaging, Endoscopes, Luminescence, Confocal microscopy, Endoscopy, Tissues, Biopsy, In vivo imaging, Tissue optics, Fluorescence resonance energy transfer

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Proceedings Article | 18 February 2011 Paper

Fluorescence lifetime imaging of skin cancer

Rakesh Patalay, Clifford Talbot, Ian Munro, Hans Georg Breunig, Karsten König, Yuri Alexandrov, Sean Warren, Mark Neil, Paul M. French, Anthony Chu, Gordon Stamp, Chris Dunsby

Proceedings Volume 7883, 78830A (2011) https://doi.org/10.1117/12.873298

KEYWORDS: Luminescence, Fluorescence lifetime imaging, Skin cancer, Tissues, Skin, Photons, Two photon excitation microscopy, Diagnostics, Photonics, Roads

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Proceedings Article | 17 February 2011 Paper

Hyperspectral fluorescence lifetime fibre probe spectroscopy for use in the study and diagnosis of osteoarthritis and skin cancer

Alex Thompson, Hugh Manning, Mikkel Brydegaard, Sergio Coda, Gordon Kennedy, Rakesh Patalay, Ulrika Waitong-Braemming, Pieter De Beule, Mark Neil, Stefan Andersson-Engels, Yoshifumi Itoh, Niels Bendsøe, Christopher Dunsby, Katarina Svanberg, Paul M. French

Proceedings Volume 7895, 78950G (2011) https://doi.org/10.1117/12.875120

KEYWORDS: Luminescence, Spectroscopy, Cartilage, Skin cancer, Tissues, In vivo imaging, Collagen, Tissue optics, Reflectivity, Diffuse reflectance spectroscopy

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Proceedings Article | 27 February 2010 Paper

Investigating fast enzyme-DNA kinetics using multidimensional fluorescence imaging and microfluidics

Tom Robinson, Hugh Manning, Christopher Dunsby, Mark A. A. Neil, Geoff Baldwin, Andrew de Mello, Paul M. W. French

Proceedings Volume 7593, 759304 (2010) https://doi.org/10.1117/12.840035

KEYWORDS: Microfluidics, Luminescence, Anisotropy, Fluorescence lifetime imaging, Fluorescence anisotropy, Polarization, Microscopes, Confocal microscopy, Computer simulations, Computational fluid dynamics

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Proceedings Article | 1 July 2009 Paper

Optically sectioned imaging by oblique plane microscopy

C. Dunsby

Proceedings Volume 7367, 73670H (2009) https://doi.org/10.1117/12.831543

KEYWORDS: Microscopy, Image processing, Luminescence, Microscopes, 3D image processing, Objectives, Imaging systems, 3D metrology, Confocal microscopy, 3D acquisition

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Proceedings Article | 12 February 2009 Paper

A study on optical modulation signal and tissue displacement in ultrasound modulated optical tomography

Rui Li, Daniel Elson, Chris Dunsby, Robert Eckersley, Meng-Xing Tang

Proceedings Volume 7177, 71772E (2009) https://doi.org/10.1117/12.809035

KEYWORDS: Modulation, Ultrasonography, Particles, Ultrasound-modulated optical tomography, Monte Carlo methods, Tissue optics, Phase shift keying, Acoustics, Scattering, Signal to noise ratio

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Proceedings Article | 13 July 2007 Paper

Applications of rapid time-gated hyperspectral FLIM: live cell imaging of membrane order and 6-D microscopy

H. Manning, D. Owen, E. Auksorius, P. de Beule, S. Oddos, C. Talbot, C. Dunsby, I. Munro, A. Magee, M. Neil, P. French

Proceedings Volume 6630, 663019 (2007) https://doi.org/10.1117/12.728479

KEYWORDS: Luminescence, Fluorescence lifetime imaging, Microscopes, Data acquisition, Charge-coupled devices, Live cell imaging, Microscopy, Confocal microscopy, Tissues, Spectral resolution

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Proceedings Article | 19 February 2007 Paper

Development of a hyperspectral fluorescence lifetime imaging microscope and its application to tissue imaging

Dylan Owen, Hugh Manning, Pieter de Beule, Clifford Talbot, Jose Requejo-Isidro, Chris Dunsby, James McGinty, Richard Benninger, Dan Elson, Ian Munro, Neil Galletly, M. Jon Lever, Gordon Stamp, Praveen Anand, Mark Neil, Paul French

Proceedings Volume 6441, 64411K (2007) https://doi.org/10.1117/12.700081

KEYWORDS: Fluorescence lifetime imaging, Luminescence, Microscopes, Tissues, Confocal microscopy, Hyperspectral imaging, Spectrographs, Cameras, Tissue optics, Sensors

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Proceedings Article | 14 February 2007 Paper

A novel hyperspectral lifetime probe for autofluorescence

Pieter De Beule, C. Dunsby, D. Owen, N. Galletly, U. Anand, C. Benham, A. Naylor, G. Stamp, P. Anand, P. French

Proceedings Volume 6433, 643303 (2007) https://doi.org/10.1117/12.700590

KEYWORDS: Luminescence, Skin, Proteins, Ultraviolet radiation, Optical filters, Mode conditioning cables, Tissues, Optical fibers, Sensors, Roads

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Proceedings Article | 8 October 2005 Paper

Fluorescence lifetime imaging microscopy using a tunable continuum source and a Nipkow disk confocal microscope

D. Grant, E. Auksorius, D. Schimpf, D. Elson, C. Dunsby, J. Requejo-Isidro, I. Munro, M. Neil, P. French, P. Courtney

Proceedings Volume 5860, 58600K (2005) https://doi.org/10.1117/12.632952

KEYWORDS: Fluorescence lifetime imaging, Confocal microscopy, Microscopes, Microscopy, Luminescence, Photonic crystal fibers, Confocal fluorescent microscopy, Multiphoton microscopy, Physics, Crystals

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Proceedings Article | 8 October 2005 Paper

Application of hyperspectral fluorescence lifetime imaging to tissue autofluorescence: arthritis

C. Talbot, R. Benninger, P. de Beule, J. Requejo-Isidro, D. Elson, C. Dunsby, I. Munro, M. Neil, A. Sandison, N. Sofat, H. Nagase, P. French, M. Lever

Proceedings Volume 5862, 58620T (2005) https://doi.org/10.1117/12.633034

KEYWORDS: Cartilage, Tissues, Fluorescence lifetime imaging, Luminescence, Head, Hyperspectral imaging, Physics, Software development, Diagnostics, Statistical analysis

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SPIE Journal Paper | 1 September 2005 Open Access

Toward the clinical application of time-domain fluorescence lifetime imaging

Ian Munro, James McGinty, Neil Galletly, Jose Requejo-Isidro, Peter Lanigan, Daniel Elson, Christopher Dunsby, Mark Neil, M. John Lever, Gordon Stamp, Paul French

JBO, Vol. 10, Issue 05, 051403, (September 2005) https://doi.org/10.1117/12.10.1117/1.2102807

KEYWORDS: Fluorescence lifetime imaging, Luminescence, Tissues, Endoscopes, Tissue optics, Endoscopy, Signal to noise ratio, Charge-coupled devices, Picosecond phenomena, Image intensifiers

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Proceedings Article | 1 September 2005 Paper

Video rate fluorescence lifetime imaging and fluorescence lifetime endoscopy

J. McGinty, I. Munro, J. Requejo-Isidro, D. Elson, C. Dunsby, M. Neil, P. French, N. Galletly, G. Stamp

Proceedings Volume 5864, 586408 (2005) https://doi.org/10.1117/12.632913

KEYWORDS: Fluorescence lifetime imaging, Endoscopes, Luminescence, Endoscopy, Tissues, Tissue optics, Video, Picosecond phenomena, Biomedical optics, Laparoscopy

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Proceedings Article | 23 September 2003 Paper

Photorefractive holography for real-time coherence gated imaging

Christopher Dunsby, Y. Gu, Z. Ansari, D. Nolte, M. Melloch, P. French

Proceedings Volume 5067, (2003) https://doi.org/10.1117/12.518500

KEYWORDS: Coherence (optics), Holography, Gated imaging, Signal detection, Video, Scattering media, Cameras, Charge-coupled devices, Optical coherence tomography, Physics

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Proceedings Article | 8 July 2003 Paper

Wide-field coherence gated imaging: photorefractive holography and wide-field coherent heterodyne imaging

Christopher Dunsby, Yan Gu, David Nolte, Michael Melloch, Paul French

Proceedings Volume 4956, (2003) https://doi.org/10.1117/12.478993

KEYWORDS: Coherence imaging, Light scattering, Charge-coupled devices, Holography, CCD cameras, Scattering, Polarization, Coherence (optics), Optical coherence tomography, Signal detection

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Proceedings Article | 8 July 2003 Paper

Imaging of tumor necroses using full-frame optical coherence imaging

Ping Yu, L. Peng, M. Mustata, David Nolte, J. Turek, Michael Melloch, Christopher Dunsby, Yan Gu, Paul French

Proceedings Volume 4956, (2003) https://doi.org/10.1117/12.477823

KEYWORDS: Tumors, Holograms, Holography, Coherence imaging, Speckle, Tissue optics, Optical coherence tomography, CCD cameras, Four wave mixing, Biomedical optics

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Proceedings Article | 14 June 2002 Paper

Low-coherence photorefractive holography for high-speed 3D imaging including through scattering media

Christopher Dunsby, Y. Gu, Zunaira Ansari, Duncan Parsons-Karavassilis, Jan Siegel, Masahide Itoh, S. Iwamoto, William Headley, Michael Melloch, David Nolte, Paul French

Proceedings Volume 4619, (2002) https://doi.org/10.1117/12.470469

KEYWORDS: Microscopes, Holography, Light scattering, Coherence (optics), Modulation, Stereoscopy, Scattering, CCD cameras, Charge-coupled devices, Mirrors

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Proceedings Article | 31 May 2002 Paper

High-speed 3-D imaging using photorefractive holography with novel low-coherence interferometers

Y. Gu, Zunaira Ansari, Christopher Dunsby, Mary Tziraki, Duncan Parsons-Karavassilis, Jan Siegel, Paul French, David Nolte, Michael Melloch

Proceedings Volume 4705, (2002) https://doi.org/10.1117/12.469019

KEYWORDS: Holography, Light scattering, Stereoscopy, 3D image reconstruction, Luminescence, Microscopes, Coherence (optics), Scattering media, Beam splitters, Prisms

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Proceedings Article | 24 October 2001 Paper

High-speed 3D imaging using photorefractive holography with novel low-coherence interferometers

Yan Gu, Zunaira Ansari, Jan Siegel, Christopher Dunsby, Masahide Itoh, Duncan Parsons-Karavassilis, Mary Tziraki, Richard Jones, Keith Dowling, Paul French, David Nolte, William Headley, Michael Melloch

Proceedings Volume 4434, (2001) https://doi.org/10.1117/12.446694

KEYWORDS: Holography, Light scattering, Modulation, Semiconductor lasers, Light emitting diodes, Imaging systems, Tissue optics, CCD cameras, Stereoscopy, Luminescence

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Showing 5 of 36 publications

Conference Committee Involvement (1)

Neurophotonics

6 April 2020 | Online Only, France

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