Photoimmunotherapy employs antibody-photosensitizer constructs (photoimmunoconjugates) for targeted cancer ablation through the generation of reactive oxygen species. While this approach enhances cancer cell specificity, it sacrifices cellular uptake. This study addresses this limitation through two strategies with an emphasis on anti-cancer immunogenicity: 1) utilizing fluid shear stress to mediate delivery, and 2) leveraging nanoengineering approaches to maximize photoimmunoconjugate payload. Results reveal that fluid shear stress promotes photosensitizer delivery and anti-tumor immune response while modulating subcellular localization. By shedding light on improved delivery strategies and formulations, this study generates important implications for the clinical implementation of photoimmunotherapy.
Ovarian cancer typically spreads throughout the peritoneal cavity, and despite standard of care treatments (surgical debulking and chemotherapy), the five-year relative survival rate remains below 50%. The use of antibody-photosensitizer conjugates (photoimmunotherapy) has emerged as a promising modality to achieve targeted photosensitizer delivery to ovarian cancer cells. In this study, we investigate epithelial growth factor (EGFR)-targeted PIT coupled with inhibition of prostaglandin E2 receptor 4 (EP4), a G-coupled-receptor that contributes to cancer progression and intracellularly transactivates EGFR. This potent triple combination significantly attenuates the metastatic behavior of ovarian cancer cells through simultaneously inducing photochemical damage and modulating protein expression.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.