Obesity is associated to a delayed wound healing and prolonged inflammatory phase. Laser light has shown positive
results in the photobiomodulation of tissue repair; however, its use associated with systemic disorders such as obesity is
still little explored in the literature. The aim of this study was to validate an experimental system for studying weight
gaining by consuming a high fat diet called "cafeteria diet" (CD) for the induction of obesity. Forty-eight rats were
weaned, divided into two experimental groups: standard diet (SD) and Cafeteria Diet (CD). Free feeding was carried out
during 20 weeks and the mass gaining was accompanied. After general anesthesia standardized surgical wounds were
created (1cm2) in the dorsal midline region of each animal. Both groups (SD; CD) were divided into 2 subgroups of 12 animals, G1 and G3 (non-irradiated) and G2 and G4 (irradiated). The irradiation protocols (λ660 nm, 40 mW, CW; 24
J/cm2) started immediately after surgery and were repeated every other day during 14 days. The rats were killed at the
8th or 15th days after surgery. The abdominal fat was removed and weighed to verify the success of the induction
technique. The specimens were taken and routinely processed histology (hematoxylin/eosin) was performed. It was
concluded that the ingestion of fast-food increased abdominal fat in rats and modified the inflammatory pattern of the
healing. Laser phototherapy in the parameters employed decreased inflammatory intensity quickening wound healing in
obese rats.
Gardênia Paraguassú, Isabele Cardoso De Castro, Rebeca Vasconcelos, Milena da Guarda, Tânia Rodriguez, Maria José Ramalho, Antônio Luiz Pinheiro, Luciana Maria Ramalho
Hypothyroidism has been associated with the disruption of the body's metabolism, including the healing
process. LED phototherapy has been studied using several healing models, but their effects on mast cells
proliferation associated to hypothyroidism remains unknown. The aim of this study was to assess the effect
LED (λ630±20nm) phototherapy on mast cells proliferation during tissue repair in hypothyroid rats. Under
general anesthesia, a standard surgical wound (1cm2) was created on the dorsum of 24 male Wistar rats
divided into 4 groups of 6 animals each: EC-Control Euthyroid; ED-Euthyroid+LED; HC-Control
Hypothyroid and HD-Hypothyroid+LED. The irradiation started immediately after surgery and was repeated
every other day for 7 days, when animals death occurred. Hypothyroidism was induced in rats with
propylthiouracil (0.05g/100mL) administered orally for 4 weeks and maintained until the end of the
experiment. The specimens removed were processed to wax and stained with toluidine blue for mast cell
identification. The mast cell proliferation was significantly higher in HC group than in EC group (Mann
Whitney, p<0.05), but when ED group was compared to HD group, no significant difference was found. Our
results showed that there was increase of mast cells in the presence of hypothyroidism, prolonging the
inflammatory phase of repair, and the LED light has a biomodulative effect on mast cell population, even
when hipothyroidism was present.
The Bell's palsy (G51) consists of a unilateral face paralysis that sudden begins with unknown cause and can result in
complete mimic loss or partial paralysis of the face. Damage to the VII cranial nerve can be found in the pathology,
promoting mussel's inactivity. The light Photobiomodulation (LPBM) has presented ability of rush the tissue repair,
favoring the regeneration of neural structures. The present study aimed to assess the effectiveness use of the 780nm laser
and 850nm LED (light-emitting diode) in the treatment of the face paralysis. Were evaluated 14 patients that suffer of
Bell's palsy whom were submitted to the light administration, on the Laser Clinic of the UFBA between 2005 and 2010.
The treatment was performed by infrared Laser in 11 patients (78.57%), and by LED in 3 patients (21.42%). At the end
of the 12 sections, 11 patients (78.57%) had presented themselves cure or with substantial improvement of the initial
picture, however 3 patients (21.42%) dealt with infra-red Laser λ780nm had not evolution. The light presented as an
effective method for the treatment of Bell's palsy, but the association with the physiotherapy and medications is
important.
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